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Retinoic Acid-induced Differentiation of Rat Mesenchymal Stem Cells into β-Cell Lineage

 Jaehyung Kim  ;  Kyungsik Kim  ;  Sangwoo Lee  ;  Hyunwoo Kim  ;  Dongjin Joo  ;  Yuseun Kim  ;  Hwal Suh 
 Journal of the Korean Society for Transplantation (대한이식학회지), Vol.29(3) : 118-129, 2015 
Journal Title
 Journal of the Korean Society for Transplantation (대한이식학회지) 
Issue Date
Backgrounds: Type I diabetes mellitus (T1DM), an autoimmune disease, is associated with insulin deficiency due to the death of β-cells. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are capable of tissue repair and thus are a promising source of β-cell surrogates. Methods: In this study, the therapeutic potential of BM-MSCs as β-cell replacements was analyzed both in vitro and in vivo. First, we used retinoic acid (RA) to induce rat BM-MSCs to differentiate into cells of endodermal/pancreatic lineage. Then, differentiated rat BM-MSCs were syngeneically injected under the renal capsule of rats. Results: Analysis of gene expression revealed that rat BM-MSCs showed signs of early pancreatic development, and differentiated cells were qualitatively and quantitatively confirmed to produce insulin in vitro. In vivo study was performed for short-term (3 weeks) and long-term (8 weeks) period of time. Rats that were injected with differentiated MSCs exhibited a reduction in blood glucose levels throughout 8 weeks, and grafted cells survived in vivo for at least 3 weeks. Conclusions: These findings show that RA can induce differentiation of MSCs into the β-cell lineage and demonstrate the potential of BM-MSCs to serve as therapeutic tools for T1DM.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sik(김경식) ORCID logo https://orcid.org/0000-0001-9498-284X
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Kim, Jae Hyung(김재형)
Kim, Hyun Woo(김현우) ORCID logo https://orcid.org/0000-0001-8576-1877
Suh, Hwal(서활)
Lee, Sang Woo(이상우)
Joo, Dong Jin(주동진) ORCID logo https://orcid.org/0000-0001-8405-1531
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