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Retinoic Acid-induced Differentiation of Rat Mesenchymal Stem Cells into β-Cell Lineage

Authors
 Jaehyung Kim  ;  Kyungsik Kim  ;  Sangwoo Lee  ;  Hyunwoo Kim  ;  Dongjin Joo  ;  Yuseun Kim  ;  Hwal Suh 
Citation
 Journal of the Korean Society for Transplantation (대한이식학회지), Vol.29(3) : 118-129, 2015 
Journal Title
 Journal of the Korean Society for Transplantation  (대한이식학회지) 
ISSN
 1598-1711 
Issue Date
2015
Abstract
Backgrounds: Type I diabetes mellitus (T1DM), an autoimmune disease, is associated with insulin deficiency due to the death of β-cells. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are capable of tissue repair and thus are a promising source of β-cell surrogates. Methods: In this study, the therapeutic potential of BM-MSCs as β-cell replacements was analyzed both in vitro and in vivo. First, we used retinoic acid (RA) to induce rat BM-MSCs to differentiate into cells of endodermal/pancreatic lineage. Then, differentiated rat BM-MSCs were syngeneically injected under the renal capsule of rats. Results: Analysis of gene expression revealed that rat BM-MSCs showed signs of early pancreatic development, and differentiated cells were qualitatively and quantitatively confirmed to produce insulin in vitro. In vivo study was performed for short-term (3 weeks) and long-term (8 weeks) period of time. Rats that were injected with differentiated MSCs exhibited a reduction in blood glucose levels throughout 8 weeks, and grafted cells survived in vivo for at least 3 weeks. Conclusions: These findings show that RA can induce differentiation of MSCs into the β-cell lineage and demonstrate the potential of BM-MSCs to serve as therapeutic tools for T1DM.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/141422
DOI
10.4285/jkstn.2015.29.3.118
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실)
Yonsei Authors
김경식(Kim, Kyung Sik) ; 김유선(Kim, Yu Seun) ; 김재형(Kim, Jae Hyung) ; 김현우(Kim, Hyun Woo) ; 서활(Suh, Hwal) ; 이상우(Lee, Sang Woo) ; 주동진(Joo, Dong Jin)
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