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A novel siderophore system is essential for the growth of Pseudomonas aeruginosa in airway mucus

 Mia Gi  ;  Kang-Mu Lee  ;  Sang Cheol Kim  ;  Joo-Heon Yoon  ;  Sang Sun Yoon  ;  Jae Young Choi 
 SCIENTIFIC REPORTS, Vol.5 : 14644, 2015 
Journal Title
Issue Date
Animals ; Azetidinecarboxylic Acid/analogs & derivatives ; Azetidinecarboxylic Acid/pharmacology ; Chlorides/pharmacology ; Coculture Techniques ; Epithelial Cells/cytology ; Epithelial Cells/drug effects ; Epithelial Cells/secretion* ; Ferric Compounds/pharmacology ; Gene Expression Regulation, Bacterial* ; Genes, Bacterial* ; Host-Pathogen Interactions ; Humans ; Iron/metabolism* ; Male ; Mice ; Mice, Inbred C57BL ; Microarray Analysis ; Microbial Viability/drug effects ; Mucus/chemistry* ; Mutation ; Oligopeptides/metabolism ; Phenols/metabolism ; Primary Cell Culture ; Pseudomonas Infections/microbiology ; Pseudomonas Infections/pathology ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/growth & development ; Pseudomonas aeruginosa/metabolism* ; Siderophores/biosynthesis ; Siderophores/deficiency ; Thiazoles/metabolism ; Trachea/cytology ; Trachea/secretion
Pseudomonasaeruginosaestablishesairwayinfections in Cystic Fibrosis patients. Here, we investigate the molecular interactions between P.aeruginosaandairwaymucussecretions (AMS) derived from the primary cultures of normal human tracheal epithelial (NHTE) cells. PAO1, a prototype strain of P.aeruginosa, was capable of proliferating during incubation with AMS, while all other tested bacterial species perished. A PAO1 mutant lacking PA4834 gene became susceptible to AMS treatment. The ΔPA4834 mutant was grown in AMS supplemented with 100 μM ferric iron, suggesting that the PA4834 gene product is involved in iron metabolism. Consistently, intracellular iron content was decreased in the mutant, but not in PAO1 after the AMS treatment. Importantly, a PAO1 mutant unable to produce both pyoverdine and pyochelin remained viable, suggesting that these two majorsiderophoremolecules are dispensable for maintaining viability during incubation with AMS. The ΔPA4834 mutant was regrown in AMS amended with 100 μM nicotianamine, a phytosiderophore whose production is predicted to be mediated by the PA4836 gene. Infectivity of the ΔPA4834 mutant was also significantly compromised in vivo. Together, our results identify a genetic element encoding anoveliron acquisitionsystemthat plays a previously undiscovered role in P.aeruginosaairwayinfection.
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1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Gi, Mi A(기미아)
Kim, Sang Cheol(김상철)
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
Yoon, Joo Heon(윤주헌)
Lee, Kang Mu(이강무) ORCID logo https://orcid.org/0000-0001-7414-5921
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
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