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(18)F-fluoride PET imaging in a nude rat model of bone metastasis from breast cancer: Comparison with (18)F-FDG and bioluminescence imaging

 Won Jun Kang  ;  Eun Hye Song  ;  Jun Young Park  ;  Young Jin Park  ;  Arthur Cho  ;  Ho-Taek Song 
 NUCLEAR MEDICINE AND BIOLOGY, Vol.42(9) : 728-733, 2015 
Journal Title
Issue Date
Animals ; Bone Neoplasms/diagnostic imaging* ; Bone Neoplasms/secondary* ; Breast Neoplasms/diagnostic imaging* ; Cell Line, Tumor ; Disease Models, Animal* ; Fluorine Radioisotopes ; Fluorodeoxyglucose F18* ; Luminescent Measurements/methods ; Positron-Emission Tomography/methods* ; Radiopharmaceuticals ; Rats ; Rats, Nude ; Reproducibility of Results ; Sensitivity and Specificity
(18)F-fluoride PET ; Bone metastasis ; Breast cancer ; FDG PET ; Nude rat model
INTRODUCTION: Clinically-relevant animal models and appropriate imaging diagnostic tools are essential to study cancer and develop novel therapeutics. We evaluated a model of bone metastasis in nude rats by micro-PET and bioluminescence imaging. METHODS: A bone metastasis model was produced by intracardiac injection of osteotropic MDA-MB-231Bo-Luc human breast cancer cells into nude rats. Bioluminescence imaging and micro-PET scans using (18)F-FDG and (18)F-fluoride were acquired serially for 5 weeks. We correlated bioluminescence imaging, (18)F-FDG and (18)F-fluoride PET images, and histological slides. RESULTS: Multiple bone metastases were successfully evaluated by bioluminescence imaging and (18)F-FDG and (18)F-fluoride PET scans. Bioluminescence photon flux increased exponentially on weekly follow-up. (18)F-FDG PET revealed increased FDG uptake at the spine and bilaterally in the hind legs in week 2 images, and showed a progressive pattern up to 4 weeks that correlated with bioluminescence imaging. (18)F-fluoride PET showed minimal abnormal findings in week 2 images, but it showed an irregular pattern at the spine from week 3 or 4 images. On quantitative analysis with standardized uptake values, a pattern of gradual increase was observed from week 2 to week 4 in both (18)F-FDG PET and fluoride PET. Histopathological examination confirmed the formation of osteolytic metastasis and necrosis of the distal femur, which appeared as a photon defect on PET scans. CONCLUSION: Developing bone metastasis from breast cancer in a nude rat model was successfully evaluated with an animal PET imaging system and bioluminescence imaging. This nude rat model of bone metastasis, which can be evaluated by PET imaging, may be a valuable tool for evaluating early responses to novel therapeutics.
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1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실) > 1. Journal Papers
Yonsei Authors
Kang, Won Jun(강원준) ORCID logo https://orcid.org/0000-0002-2107-8160
Park, Vivian Youngjean(박영진) ORCID logo https://orcid.org/0000-0002-5135-4058
Song, Eun Hye(송은혜)
Song, Ho Taek(송호택) ORCID logo https://orcid.org/0000-0002-6655-2575
Cho, Arthur Eung Hyuck(조응혁) ORCID logo https://orcid.org/0000-0001-8670-2473
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