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Ectopic Hard Tissue Formation by Odonto/Osteogenically In Vitro Differentiated Human Deciduous Teeth Pulp Stem Cells

Authors
 Seunghye Kim  ;  Je Seon Song  ;  Mijeong Jeon  ;  Dong Min Shin  ;  Seong-Oh Kim  ;  Jae Ho Lee 
Citation
 Calcified Tissue International, Vol.97(1) : 80-89, 2015 
Journal Title
 Calcified Tissue International 
ISSN
 0171-967X 
Issue Date
2015
Abstract
There have been many attempts to use the pulp tissue from human deciduous teeth for dentin or bone regeneration. The objective of this study was to determine the effects of odonto/osteogenic in vitro differentiation of deciduous teeth pulp stem cells (DTSCs) on their in vivo hard tissue-forming potential. DTSCs were isolated from extracted deciduous teeth using the outgrowth method. These cells were exposed to odonto/osteogenic stimuli for 4 and 8 days (Day 4 and Day 8 groups, respectively), while cells in the control group were cultured in normal medium. The in vitro differentiated DTSCs and the control DTSCs were transplanted subcutaneously into immunocompromised mice with macroporous biphasic calcium phosphate and sacrificed at 8 weeks post-implantation. The effect of odonto/osteogenic in vitro differentiation was evaluated using alkaline phosphatase (ALP) staining and quantitative reverse transcription polymerase chain reaction (RT-PCR). The in vivo effect was evaluated by qualitative RT-PCR, assessment of ALP activity, histologic analysis, and immunohistochemical staining. The amount of hard tissue was greater in Day 4 group than Day 8 group (p = 0.014). However, Day 8 group generated lamellar bone-like structure, which was immunonegative to anti-human dentin sialoprotein with significantly low expression level of DSPP compared with the control group (p = 0.008). This study demonstrates that odonto/osteogenic in vitro differentiation of DTSCs enhances the formation of bone-like tissue, instead of dentin-like tissue, when transplanted subcutaneously using MBCP as a carrier. The odonto/osteogenic in vitro differentiation of DTSCs may be an effective modification that enhances in vivo bone formation by DTSCs.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140651
DOI
10.1007/s00223-015-9989-1
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실)
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실)
Yonsei Authors
김성오(Kim, Seong Oh) ; 김승혜(Kim, Seung Hye) ; 송제선(Song, Je Seon) ; 신동민(Shin, Dong Min) ; 이제호(Lee, Jae Ho) ; 전미정(Jeon, Mi Jeong)
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Full Text
http://link.springer.com/article/10.1007%2Fs00223-015-9989-1
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