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In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration

 Jung U Shin  ;  Ji Yeon Noh  ;  Ju Hee Lee  ;  Won Jai Lee  ;  Jong Shin Yoo  ;  Jin Young Kim  ;  Hyeran Kim  ;  Inhee Jung  ;  Shan Jin  ;  Kwang Hoon Lee 
 EXPERIMENTAL DERMATOLOGY, Vol.24(6) : 478-480, 2015 
Journal Title
Issue Date
Cell Line ; Cell Movement/drug effects* ; Cell Movement/physiology ; Dose-Response Relationship, Drug ; Estrogens/pharmacology* ; Gene Expression Regulation/drug effects ; Gene Expression Regulation/genetics ; Gene Knockdown Techniques ; HMGB1 Protein/drug effects ; HMGB1 Protein/genetics ; HMGB1 Protein/physiology* ; Humans ; Keratinocytes/cytology ; Keratinocytes/drug effects* ; Keratinocytes/physiology ; Proteomics/methods* ; RNA, Small Interfering/genetics ; RNA, Small Interfering/pharmacology ; Signal Transduction/drug effects ; Signal Transduction/physiology ; Time Factors
HMGB1 ; estrogen ; keratinocyte
It is known that oestrogen influences skin wound healing by modulating the inflammatory response, cytokine expression and extracellular matrix deposition; accelerating re-epithelialization; and stimulating angiogenesis. To identify novel proteins associated with effects of oestrogen on keratinocyte, stable isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry was performed. Using SILAC, quantification of 1085 proteins was achieved. Among these proteins, 60 proteins were upregulated and 32 proteins were downregulated. Among significantly upregulated proteins, high-mobility group protein B1 (HMGB1) has been further evaluated for its role in the effect of oestrogen on keratinocytes. HMGB1 expression was strongly induced in oestrogen-treated keratinocytes in dose- and time-dependent manner. Further, HMGB1 was able to significantly accelerate the rate of HaCaT cell migration. To determine whether HMGB1 is involved in E2-induced HaCaT cell migration, cells were transfected with HMGB1 siRNA. Knockdown of HMGB1 blocked oestrogen-induced keratinocyte migration. Collectively, these experiments demonstrate that HMGB1 is a novel downstream mediator of oestrogen-stimulated keratinocyte migration.
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1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Jin, Shan(김산)
Noh, Ji Yeon(노지연)
Shin, Jung U(신정우) ORCID logo https://orcid.org/0000-0001-5259-6879
Lee, Kwang Hoon(이광훈)
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
Jung, Inhee(정인희)
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