Cited 6 times in
In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration
DC Field | Value | Language |
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dc.contributor.author | 김산 | - |
dc.contributor.author | 노지연 | - |
dc.contributor.author | 신정우 | - |
dc.contributor.author | 이광훈 | - |
dc.contributor.author | 이원재 | - |
dc.contributor.author | 이주희 | - |
dc.contributor.author | 정인희 | - |
dc.date.accessioned | 2016-02-04T11:30:31Z | - |
dc.date.available | 2016-02-04T11:30:31Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0906-6705 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/140582 | - |
dc.description.abstract | It is known that oestrogen influences skin wound healing by modulating the inflammatory response, cytokine expression and extracellular matrix deposition; accelerating re-epithelialization; and stimulating angiogenesis. To identify novel proteins associated with effects of oestrogen on keratinocyte, stable isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry was performed. Using SILAC, quantification of 1085 proteins was achieved. Among these proteins, 60 proteins were upregulated and 32 proteins were downregulated. Among significantly upregulated proteins, high-mobility group protein B1 (HMGB1) has been further evaluated for its role in the effect of oestrogen on keratinocytes. HMGB1 expression was strongly induced in oestrogen-treated keratinocytes in dose- and time-dependent manner. Further, HMGB1 was able to significantly accelerate the rate of HaCaT cell migration. To determine whether HMGB1 is involved in E2-induced HaCaT cell migration, cells were transfected with HMGB1 siRNA. Knockdown of HMGB1 blocked oestrogen-induced keratinocyte migration. Collectively, these experiments demonstrate that HMGB1 is a novel downstream mediator of oestrogen-stimulated keratinocyte migration. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 478~480 | - |
dc.relation.isPartOf | EXPERIMENTAL DERMATOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Movement/drug effects* | - |
dc.subject.MESH | Cell Movement/physiology | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Estrogens/pharmacology* | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | Gene Expression Regulation/genetics | - |
dc.subject.MESH | Gene Knockdown Techniques | - |
dc.subject.MESH | HMGB1 Protein/drug effects | - |
dc.subject.MESH | HMGB1 Protein/genetics | - |
dc.subject.MESH | HMGB1 Protein/physiology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Keratinocytes/cytology | - |
dc.subject.MESH | Keratinocytes/drug effects* | - |
dc.subject.MESH | Keratinocytes/physiology | - |
dc.subject.MESH | Proteomics/methods* | - |
dc.subject.MESH | RNA, Small Interfering/genetics | - |
dc.subject.MESH | RNA, Small Interfering/pharmacology | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Signal Transduction/physiology | - |
dc.subject.MESH | Time Factors | - |
dc.title | In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학) | - |
dc.contributor.googleauthor | Jung U Shin | - |
dc.contributor.googleauthor | Ji Yeon Noh | - |
dc.contributor.googleauthor | Ju Hee Lee | - |
dc.contributor.googleauthor | Won Jai Lee | - |
dc.contributor.googleauthor | Jong Shin Yoo | - |
dc.contributor.googleauthor | Jin Young Kim | - |
dc.contributor.googleauthor | Hyeran Kim | - |
dc.contributor.googleauthor | Inhee Jung | - |
dc.contributor.googleauthor | Shan Jin | - |
dc.contributor.googleauthor | Kwang Hoon Lee | - |
dc.identifier.doi | 10.1111/exd.12713 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00519 | - |
dc.contributor.localId | A01296 | - |
dc.contributor.localId | A02149 | - |
dc.contributor.localId | A03005 | - |
dc.contributor.localId | A03699 | - |
dc.contributor.localId | A03171 | - |
dc.contributor.localId | A02674 | - |
dc.relation.journalcode | J00866 | - |
dc.identifier.eissn | 1600-0625 | - |
dc.identifier.pmid | 25828589 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/exd.12713/abstract | - |
dc.subject.keyword | HMGB1 | - |
dc.subject.keyword | estrogen | - |
dc.subject.keyword | keratinocyte | - |
dc.contributor.alternativeName | Jin, Shan | - |
dc.contributor.alternativeName | Noh, Ji Yeon | - |
dc.contributor.alternativeName | Shin, Jung U | - |
dc.contributor.alternativeName | Lee, Kwang Hoon | - |
dc.contributor.alternativeName | Lee, Won Jai | - |
dc.contributor.alternativeName | Lee, Ju Hee | - |
dc.contributor.alternativeName | Jung, Inhee | - |
dc.contributor.affiliatedAuthor | Jin, Shan | - |
dc.contributor.affiliatedAuthor | Noh, Ji Yeon | - |
dc.contributor.affiliatedAuthor | Shin, Jung U | - |
dc.contributor.affiliatedAuthor | Lee, Won Jai | - |
dc.contributor.affiliatedAuthor | Jung, Inhee | - |
dc.contributor.affiliatedAuthor | Lee, Ju Hee | - |
dc.contributor.affiliatedAuthor | Lee, Kwang Hoon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 24 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 478 | - |
dc.citation.endPage | 480 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL DERMATOLOGY, Vol.24(6) : 478-480, 2015 | - |
dc.identifier.rimsid | 30184 | - |
dc.type.rims | ART | - |
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