1 288

Cited 4 times in

In vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration

DC FieldValueLanguage
dc.contributor.author김산-
dc.contributor.author노지연-
dc.contributor.author신정우-
dc.contributor.author이광훈-
dc.contributor.author이원재-
dc.contributor.author이주희-
dc.contributor.author정인희-
dc.date.accessioned2016-02-04T11:30:31Z-
dc.date.available2016-02-04T11:30:31Z-
dc.date.issued2015-
dc.identifier.issn0906-6705-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140582-
dc.description.abstractIt is known that oestrogen influences skin wound healing by modulating the inflammatory response, cytokine expression and extracellular matrix deposition; accelerating re-epithelialization; and stimulating angiogenesis. To identify novel proteins associated with effects of oestrogen on keratinocyte, stable isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry was performed. Using SILAC, quantification of 1085 proteins was achieved. Among these proteins, 60 proteins were upregulated and 32 proteins were downregulated. Among significantly upregulated proteins, high-mobility group protein B1 (HMGB1) has been further evaluated for its role in the effect of oestrogen on keratinocytes. HMGB1 expression was strongly induced in oestrogen-treated keratinocytes in dose- and time-dependent manner. Further, HMGB1 was able to significantly accelerate the rate of HaCaT cell migration. To determine whether HMGB1 is involved in E2-induced HaCaT cell migration, cells were transfected with HMGB1 siRNA. Knockdown of HMGB1 blocked oestrogen-induced keratinocyte migration. Collectively, these experiments demonstrate that HMGB1 is a novel downstream mediator of oestrogen-stimulated keratinocyte migration.-
dc.description.statementOfResponsibilityopen-
dc.format.extent478~480-
dc.relation.isPartOfEXPERIMENTAL DERMATOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHCell Line-
dc.subject.MESHCell Movement/drug effects*-
dc.subject.MESHCell Movement/physiology-
dc.subject.MESHDose-Response Relationship, Drug-
dc.subject.MESHEstrogens/pharmacology*-
dc.subject.MESHGene Expression Regulation/drug effects-
dc.subject.MESHGene Expression Regulation/genetics-
dc.subject.MESHGene Knockdown Techniques-
dc.subject.MESHHMGB1 Protein/drug effects-
dc.subject.MESHHMGB1 Protein/genetics-
dc.subject.MESHHMGB1 Protein/physiology*-
dc.subject.MESHHumans-
dc.subject.MESHKeratinocytes/cytology-
dc.subject.MESHKeratinocytes/drug effects*-
dc.subject.MESHKeratinocytes/physiology-
dc.subject.MESHProteomics/methods*-
dc.subject.MESHRNA, Small Interfering/genetics-
dc.subject.MESHRNA, Small Interfering/pharmacology-
dc.subject.MESHSignal Transduction/drug effects-
dc.subject.MESHSignal Transduction/physiology-
dc.subject.MESHTime Factors-
dc.titleIn vivo relative quantitative proteomics reveals HMGB1 as a downstream mediator of oestrogen-stimulated keratinocyte migration-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Dermatology (피부과학)-
dc.contributor.googleauthorJung U Shin-
dc.contributor.googleauthorJi Yeon Noh-
dc.contributor.googleauthorJu Hee Lee-
dc.contributor.googleauthorWon Jai Lee-
dc.contributor.googleauthorJong Shin Yoo-
dc.contributor.googleauthorJin Young Kim-
dc.contributor.googleauthorHyeran Kim-
dc.contributor.googleauthorInhee Jung-
dc.contributor.googleauthorShan Jin-
dc.contributor.googleauthorKwang Hoon Lee-
dc.identifier.doi10.1111/exd.12713-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00519-
dc.contributor.localIdA01296-
dc.contributor.localIdA02149-
dc.contributor.localIdA03005-
dc.contributor.localIdA03699-
dc.contributor.localIdA03171-
dc.contributor.localIdA02674-
dc.relation.journalcodeJ00866-
dc.identifier.eissn1600-0625-
dc.identifier.pmid25828589-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/exd.12713/abstract-
dc.subject.keywordHMGB1-
dc.subject.keywordestrogen-
dc.subject.keywordkeratinocyte-
dc.contributor.alternativeNameJin, Shan-
dc.contributor.alternativeNameNoh, Ji Yeon-
dc.contributor.alternativeNameShin, Jung U-
dc.contributor.alternativeNameLee, Kwang Hoon-
dc.contributor.alternativeNameLee, Won Jai-
dc.contributor.alternativeNameLee, Ju Hee-
dc.contributor.alternativeNameJung, Inhee-
dc.contributor.affiliatedAuthorJin, Shan-
dc.contributor.affiliatedAuthorNoh, Ji Yeon-
dc.contributor.affiliatedAuthorShin, Jung U-
dc.contributor.affiliatedAuthorLee, Won Jai-
dc.contributor.affiliatedAuthorJung, Inhee-
dc.contributor.affiliatedAuthorLee, Ju Hee-
dc.contributor.affiliatedAuthorLee, Kwang Hoon-
dc.rights.accessRightsnot free-
dc.citation.volume24-
dc.citation.number6-
dc.citation.startPage478-
dc.citation.endPage480-
dc.identifier.bibliographicCitationEXPERIMENTAL DERMATOLOGY, Vol.24(6) : 478-480, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.