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HMGB1 Binds to Lipoteichoic Acid and Enhances TNF-α and IL-6 Production through HMGB1-Mediated Transfer of Lipoteichoic Acid to CD14 and TLR2.

Authors
 Kwak M.S.  ;  Lim M.  ;  Lee Y.J.  ;  Lee H.S.  ;  Kim Y.H.  ;  Youn J.H.  ;  Choi J.E.  ;  Shin J.-S. 
Citation
 JOURNAL OF INNATE IMMUNITY, Vol.7(4) : 405-416, 2015 
Journal Title
 JOURNAL OF INNATE IMMUNITY 
ISSN
 1662-811X 
Issue Date
2015
MeSH
HEK293 Cells ; HMGB1 Protein/genetics ; HMGB1 Protein/immunology* ; HMGB1 Protein/metabolism ; Humans ; Interleukin-6/genetics ; Interleukin-6/immunology* ; Interleukin-6/metabolism ; Lipopolysaccharide Receptors/genetics ; Lipopolysaccharide Receptors/immunology* ; Lipopolysaccharides/genetics ; Lipopolysaccharides/immunology* ; Lipopolysaccharides/metabolism ; Protein Binding/genetics ; Protein Binding/immunology ; Teichoic Acids/genetics ; Teichoic Acids/immunology* ; Teichoic Acids/metabolism ; Toll-Like Receptor 2/genetics ; Toll-Like Receptor 2/immunology* ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/immunology* ; Tumor Necrosis Factor-alpha/metabolism
Keywords
HMGB1 ; Gram-positive bacteria ; Lipoteichoic acid ; TLR2 ; Inflammation
Abstract
Lipoteichoic acid (LTA) is a component of the cell wall of Gram-positive bacteria and induces a toll-like receptor 2 (TLR2)-mediated inflammatory response upon initial binding to lipopolysaccharide-binding protein (LBP) and subsequent transfer to CD14. In this study, we identified a novel role for the nuclear protein high-mobility group box 1 (HMGB1) in LTA-mediated inflammation. Results of ELISA, surface plasmon resonance and native PAGE electrophoretic mobility shift analyses indicated that HMGB1 binds to LTA in a concentration-dependent manner and that this binding is inhibited by LBP. Native PAGE, fluorescence-based transfer and confocal imaging analyses indicated that HMGB1 catalytically disaggregates LTA and transfers LTA to CD14. NF-κB p65 nuclear transmigration, degradation of IκBα and reporter assay results demonstrated that NF-κB activity in HEK293-hTLR2/6 cells is significantly upregulated by a mixture of LTA and soluble CD14 in the presence of HMGB1. Furthermore, the production of TNF-α and IL-6 in J774A.1 and RAW264.7 cells increased significantly following treatment with a mixture of LTA and HMGB1 compared with treatment with LTA or HMGB1 alone. Thus, we propose that HMGB1 plays an important role in LTA-mediated inflammation by binding to and transferring LTA to CD14, which is subsequently transferred to TLR2 to induce an inflammatory response.
Full Text
http://www.karger.com/Article/FullText/369972
DOI
10.1159/000369972
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kwak, Man Sup(곽만섭) ORCID logo https://orcid.org/0000-0002-3989-3016
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140536
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