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Rh D blood group conversion using transcription activator-like effector nucleases

Authors
 Young-Hoon Kim  ;  Hyun O. Kim  ;  Eun J. Baek  ;  Ryo Kurita  ;  Hyuk-Jin Cha  ;  Yukio Nakamura  ;  Hyongbum Kim 
Citation
 NATURE COMMUNICATIONS, Vol.6 : 7451, 2015 
Journal Title
 NATURE COMMUNICATIONS 
Issue Date
2015
MeSH
Deoxyribonucleases/genetics* ; Erythrocytes/metabolism* ; Erythroid Precursor Cells/metabolism* ; Fetal Blood ; Gene Knockout Techniques/methods* ; Gene Transfer Techniques* ; Humans ; Induced Pluripotent Stem Cells ; Plasmids ; RNA, Messenger/metabolism* ; Reverse Transcriptase Polymerase Chain Reaction ; Rh-Hr Blood-Group System/genetics* ; Rh-Hr Blood-Group System/metabolism ; Transfection
Abstract
Group O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a 12-transmembrane domain protein. Here we convert Rh D-positive erythroid progenitor cells into D-negative cells using RHD-targeting transcription activator-like effector nucleases (TALENs). After transfection of TALEN-encoding plasmids, RHD-knockout clones are obtained. Erythroid-lineage cells differentiated from these knockout erythroid progenitor cells do not agglutinate in the presence of anti-D reagents and do not express D antigen, as assessed using flow cytometry. Our programmable nuclease-induced blood group conversion opens new avenues for compatible donor cell generation in transfusion medicine.
Files in This Item:
T201502196.pdf Download
DOI
10.1038/ncomms8451
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
Kim, Hyongbum(김형범) ORCID logo https://orcid.org/0000-0002-4693-738X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140500
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