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Rh D blood group conversion using transcription activator-like effector nucleases

DC FieldValueLanguage
dc.contributor.author김현옥-
dc.contributor.author김형범-
dc.date.accessioned2016-02-04T11:28:23Z-
dc.date.available2016-02-04T11:28:23Z-
dc.date.issued2015-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140500-
dc.description.abstractGroup O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a 12-transmembrane domain protein. Here we convert Rh D-positive erythroid progenitor cells into D-negative cells using RHD-targeting transcription activator-like effector nucleases (TALENs). After transfection of TALEN-encoding plasmids, RHD-knockout clones are obtained. Erythroid-lineage cells differentiated from these knockout erythroid progenitor cells do not agglutinate in the presence of anti-D reagents and do not express D antigen, as assessed using flow cytometry. Our programmable nuclease-induced blood group conversion opens new avenues for compatible donor cell generation in transfusion medicine.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfNATURE COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHDeoxyribonucleases/genetics*-
dc.subject.MESHErythrocytes/metabolism*-
dc.subject.MESHErythroid Precursor Cells/metabolism*-
dc.subject.MESHFetal Blood-
dc.subject.MESHGene Knockout Techniques/methods*-
dc.subject.MESHGene Transfer Techniques*-
dc.subject.MESHHumans-
dc.subject.MESHInduced Pluripotent Stem Cells-
dc.subject.MESHPlasmids-
dc.subject.MESHRNA, Messenger/metabolism*-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHRh-Hr Blood-Group System/genetics*-
dc.subject.MESHRh-Hr Blood-Group System/metabolism-
dc.subject.MESHTransfection-
dc.titleRh D blood group conversion using transcription activator-like effector nucleases-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorYoung-Hoon Kim-
dc.contributor.googleauthorHyun O. Kim-
dc.contributor.googleauthorEun J. Baek-
dc.contributor.googleauthorRyo Kurita-
dc.contributor.googleauthorHyuk-Jin Cha-
dc.contributor.googleauthorYukio Nakamura-
dc.contributor.googleauthorHyongbum Kim-
dc.identifier.doi10.1038/ncomms8451-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01122-
dc.contributor.localIdA01148-
dc.relation.journalcodeJ02293-
dc.identifier.eissn2041-1723-
dc.identifier.pmid26078220-
dc.contributor.alternativeNameKim, Hyun Ok-
dc.contributor.alternativeNameKim, Hyongbum-
dc.contributor.affiliatedAuthorKim, Hyun Ok-
dc.contributor.affiliatedAuthorKim, Hyongbum-
dc.rights.accessRightsfree-
dc.citation.volume6-
dc.citation.startPage7451-
dc.identifier.bibliographicCitationNATURE COMMUNICATIONS, Vol.6 : 7451, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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