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In Situ Recruitment of Human Bone Marrow-Derived Mesenchymal Stem Cells Using Chemokines for Articular Cartilage Regeneration

 Park, Min Sung  ;  Kim, Yun Hee  ;  Jung, Youngmee  ;  Kim, Soo Hyun  ;  Park, Jong Chul  ;  Yoon, Dong Suk  ;  Kim, Sung-Hwan  ;  Lee, Jin Woo 
 CELL TRANSPLANTATION, Vol.24(6) : 1067-1083, 2015 
Journal Title
Issue Date
Adolescent ; Adult ; Animals ; Bone Marrow Cells/cytology* ; Bone Marrow Cells/drug effects ; Bone Marrow Cells/metabolism ; Cartilage, Articular/cytology ; Cartilage, Articular/drug effects ; Cartilage, Articular/physiology* ; Cell Differentiation/drug effects ; Chemokines/pharmacology* ; Chemotaxis/drug effects ; Chondrogenesis/drug effects ; Disease Models, Animal ; Dogs ; Female ; Humans ; Inflammation Mediators/metabolism ; Leukocytes/cytology ; Leukocytes/drug effects ; Male ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/drug effects ; Mesenchymal Stromal Cells/metabolism ; Middle Aged ; Osteogenesis/drug effects ; Receptors, Chemokine/metabolism ; Regeneration/drug effects* ; Reproducibility of Results ; Tissue Scaffolds/chemistry ; Young Adult
Chemokines ; IL-8 ; MIP-3α ; Mesenchymal stem cells
Bone marrow-derived mesenchymal stem cells (BMSCs) are a good cell source for regeneration of cartilage as they can migrate directly to the site of cartilage injury and differentiate into articular chondrocytes. Articular cartilage defects do not heal completely due to the lack of chondrocytes or BMSCs at the site of injury. In this study, the chemotaxis of BMSCs toward chemokines, which may give rise to a complete regeneration of the articular cartilage, was investigated. CCR2, CCR4, CCR6, CXCR1, and CXCR2 were expressed in normal BMSCs and were increased significantly upon treatment with proinflammatory cytokines. BMSC migration was increased by MIP-3α and IL-8 more than by MCP-1 or SDF-1α. IL-8 and MIP-3α significantly enhanced the chemotaxis of BMSCs compared with MCP-1, SDF-1α, or PBS. Human BMSC recruitment to transplanted scaffolds containing either IL-8 or MIP-3α significantly increased in vivo compared to scaffolds containing PBS. Furthermore, IL-8- and MIP-3α-containing scaffolds enhanced tissue regeneration of an osteochondral defect site in beagle knee articular cartilage. Therefore, this study suggests that IL-8 and MIP-3α are the candidates that induce the regeneration of damaged articular cartilage.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Sung Hwan(김성환) ORCID logo https://orcid.org/0000-0001-5743-6241
Park, Min Sung(박민성)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
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