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Impact of hyperthermia before and during ischemia-reperfusion on neuronal damage and gliosis in the gerbil hippocampus induced by transient cerebral ischemia

Authors
 Min Joung Kim  ;  Jun Hwi Cho  ;  Jeong Hwi Cho  ;  Joon Ha Park  ;  Ji Hyeon Ahn  ;  Hyun Jin Tae  ;  Geum Sil Cho  ;  Bing Chun Yan  ;  In Koo Hwang  ;  Choong Hyun Lee  ;  Eun Joo Bae  ;  Moo Ho Won  ;  Jae Chul Lee 
Citation
 JOURNAL OF THE NEUROLOGICAL SCIENCES, Vol.348(1-2) : 101-110, 2015 
Journal Title
JOURNAL OF THE NEUROLOGICAL SCIENCES
ISSN
 0022-510X 
Issue Date
2015
MeSH
Animals ; Cell Death* ; Disease Models, Animal ; Fever/complications ; Fever/pathology* ; Gerbillinae ; Gliosis/etiology ; Gliosis/pathology* ; Hippocampus/pathology* ; Ischemic Attack, Transient/chemically induced ; Ischemic Attack, Transient/pathology* ; Male ; Pyramidal Cells/pathology* ; Random Allocation ; Reperfusion Injury/chemically induced ; Reperfusion Injury/pathology*
Keywords
Delayed neuronal death ; Glial cells ; Hippocampal subregions ; Hyperthermic pre-condition ; Ischemia–reperfusion ; Pyramidal neurons
Abstract
Hyperthermia can exacerbate the brain damage produced by ischemia. In the present study, we investigated the effects of hyperthermia before and during ischemia-reperfusion on neuronal damage and glial changes in the gerbil hippocampus following transient cerebral ischemia using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining. The animals were randomly assigned to 4 groups: (1) sham-operated animals with normothermia (normothermia + sham group); (2) ischemia-operated animals with normothermia (normothermia + ischemia group); (3) sham-operated animals with hyperthermia (hyperthermia + sham group); and (4) ischemia-operated animals with hyperthermia (hyperthermia + ischemia group). Hyperthermia (39.5 ± 0.2°C) was induced by exposing the gerbils to a heating pad connected to a rectal thermistor for 30 min before and during ischemia-reperfusion. In the normothermia+ischemia groups, a significant delayed neuronal death was observed in the stratum pyramidale (SP) of the hippocampal CA1 region (CA1) 5 days after ischemia-reperfusion. In the hyperthermia+ischemia groups, neuronal death in the SP of the CA1 occurred at 1 day post-ischemia, and neuronal death was observed in the SP of the CA2/3 region at 2 days post-ischemia. In addition, we examined activations of astrocytes and microglia using immunohistochemistry for anti-glial fibrillary acidic protein (GFAP) and anti-ionized calcium-binding adapter molecule 1 (Iba-1). GFAP-positive astrocytes and Iba-1-positive microglia in the ischemic hippocampus were activated much earlier and much more accelerated in the hyperthermia+ischemia groups than those in the normothermia+ischemia groups. Based on our findings, we suggest that an experimentally hyperthermic pre-condition before cerebral ischemic insult produces more extensive neuronal damage and glial activation in the ischemic hippocampus.
Full Text
http://www.sciencedirect.com/science/article/pii/S0022510X14007291
DOI
10.1016/j.jns.2014.11.015
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Min Joung(김민정) ORCID logo https://orcid.org/0000-0003-1634-5209
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140349
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