0 428

Cited 18 times in

Impact of hyperthermia before and during ischemia-reperfusion on neuronal damage and gliosis in the gerbil hippocampus induced by transient cerebral ischemia

DC Field Value Language
dc.contributor.author김민정-
dc.date.accessioned2016-02-04T11:24:16Z-
dc.date.available2016-02-04T11:24:16Z-
dc.date.issued2015-
dc.identifier.issn0022-510X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140349-
dc.description.abstractHyperthermia can exacerbate the brain damage produced by ischemia. In the present study, we investigated the effects of hyperthermia before and during ischemia-reperfusion on neuronal damage and glial changes in the gerbil hippocampus following transient cerebral ischemia using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining. The animals were randomly assigned to 4 groups: (1) sham-operated animals with normothermia (normothermia + sham group); (2) ischemia-operated animals with normothermia (normothermia + ischemia group); (3) sham-operated animals with hyperthermia (hyperthermia + sham group); and (4) ischemia-operated animals with hyperthermia (hyperthermia + ischemia group). Hyperthermia (39.5 ± 0.2°C) was induced by exposing the gerbils to a heating pad connected to a rectal thermistor for 30 min before and during ischemia-reperfusion. In the normothermia+ischemia groups, a significant delayed neuronal death was observed in the stratum pyramidale (SP) of the hippocampal CA1 region (CA1) 5 days after ischemia-reperfusion. In the hyperthermia+ischemia groups, neuronal death in the SP of the CA1 occurred at 1 day post-ischemia, and neuronal death was observed in the SP of the CA2/3 region at 2 days post-ischemia. In addition, we examined activations of astrocytes and microglia using immunohistochemistry for anti-glial fibrillary acidic protein (GFAP) and anti-ionized calcium-binding adapter molecule 1 (Iba-1). GFAP-positive astrocytes and Iba-1-positive microglia in the ischemic hippocampus were activated much earlier and much more accelerated in the hyperthermia+ischemia groups than those in the normothermia+ischemia groups. Based on our findings, we suggest that an experimentally hyperthermic pre-condition before cerebral ischemic insult produces more extensive neuronal damage and glial activation in the ischemic hippocampus.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF THE NEUROLOGICAL SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Death*-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHFever/complications-
dc.subject.MESHFever/pathology*-
dc.subject.MESHGerbillinae-
dc.subject.MESHGliosis/etiology-
dc.subject.MESHGliosis/pathology*-
dc.subject.MESHHippocampus/pathology*-
dc.subject.MESHIschemic Attack, Transient/chemically induced-
dc.subject.MESHIschemic Attack, Transient/pathology*-
dc.subject.MESHMale-
dc.subject.MESHPyramidal Cells/pathology*-
dc.subject.MESHRandom Allocation-
dc.subject.MESHReperfusion Injury/chemically induced-
dc.subject.MESHReperfusion Injury/pathology*-
dc.titleImpact of hyperthermia before and during ischemia-reperfusion on neuronal damage and gliosis in the gerbil hippocampus induced by transient cerebral ischemia-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Radiology (영상의학)-
dc.contributor.googleauthorMin Joung Kim-
dc.contributor.googleauthorJun Hwi Cho-
dc.contributor.googleauthorJeong Hwi Cho-
dc.contributor.googleauthorJoon Ha Park-
dc.contributor.googleauthorJi Hyeon Ahn-
dc.contributor.googleauthorHyun Jin Tae-
dc.contributor.googleauthorGeum Sil Cho-
dc.contributor.googleauthorBing Chun Yan-
dc.contributor.googleauthorIn Koo Hwang-
dc.contributor.googleauthorChoong Hyun Lee-
dc.contributor.googleauthorEun Joo Bae-
dc.contributor.googleauthorMoo Ho Won-
dc.contributor.googleauthorJae Chul Lee-
dc.identifier.doi10.1016/j.jns.2014.11.015-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00473-
dc.relation.journalcodeJ01897-
dc.identifier.eissn1878-5883-
dc.identifier.pmid25434714-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0022510X14007291-
dc.subject.keywordDelayed neuronal death-
dc.subject.keywordGlial cells-
dc.subject.keywordHippocampal subregions-
dc.subject.keywordHyperthermic pre-condition-
dc.subject.keywordIschemia–reperfusion-
dc.subject.keywordPyramidal neurons-
dc.contributor.alternativeNameKim, Min Jung-
dc.contributor.affiliatedAuthorKim, Min Jung-
dc.rights.accessRightsnot free-
dc.citation.volume348-
dc.citation.number1-2-
dc.citation.startPage101-
dc.citation.endPage110-
dc.identifier.bibliographicCitationJOURNAL OF THE NEUROLOGICAL SCIENCES, Vol.348(1-2) : 101-110, 2015-
dc.identifier.rimsid51572-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.