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Fluorescent iodized emulsion for pre- and intraoperative sentinel lymph node imaging: validation in a preclinical model

Authors
 Honsoul Kim  ;  Sang Kil Lee  ;  Yoo Min Kim  ;  Eun-Hye Lee  ;  Soo-Jeong Lim  ;  Se Hoon Kim  ;  Jaemoon Yang  ;  Joon Seok Lim  ;  Woo Jin Hyung 
Citation
 Radiology, Vol.275(1) : 196-204, 2015 
Journal Title
 Radiology 
ISSN
 0033-8419 
Issue Date
2015
MeSH
Animals ; Disease Models, Animal ; Dogs ; Emulsions/chemistry ; Emulsions/pharmacokinetics* ; Ethiodized Oil/chemistry ; Ethiodized Oil/pharmacokinetics* ; Fluorescent Dyes ; Gastrectomy ; Hexoses/chemistry ; Hexoses/pharmacokinetics ; Intraoperative Care ; Laparoscopy ; Lymph Node Excision ; Lymphography/methods* ; Male ; Polysorbates/chemistry ; Polysorbates/pharmacokinetics ; Radiographic Image Interpretation, Computer-Assisted ; Rats ; Rats, Sprague-Dawley ; Robotics ; Sentinel Lymph Node Biopsy* ; Stomach Neoplasms/diagnostic imaging ; Stomach Neoplasms/pathology* ; Stomach Neoplasms/surgery ; Surface-Active Agents/chemistry ; Surface-Active Agents/pharmacokinetics ; Tomography, X-Ray Computed/methods*
Abstract
PURPOSE: To validate the usefulness of a newly developed tracer for preoperative gastric sentinel lymph node (LN) (SLN) mapping and intraoperative navigation after a single preoperative submucosal injection in rat and beagle models. MATERIALS AND METHODS: This study was approved by the Experimental Animal Ethical Committee of Yonsei University College of Medicine according to the eighth edition of the Guide for the Care and Use of Laboratory Animals published in 2011. An emulsion was developed that contained indocyanine green in iodized oil, which can be visualized with both computed tomography (CT) and near-infrared (NIR) optical imaging and has the property of delayed washout. This emulsion was injected into the footpad of rats (n = 6) and the gastric submucosa of beagles (n = 8). CT lymphography was performed. The degree of enhancement of popliteal LNs was measured in rats, and the enhancing LNs were identified and the degree of enhancement of the enhancing LNs was measured in beagles. Next, NIR imaging was performed in beagles during open, laparoscopic, and robotic surgery to identify LNs containing the fluorescent signals of indocyanine green. The enhanced LNs detected with CT lymphography and NIR imaging were matched to see if they corresponded. RESULTS: Preoperative CT lymphography facilitated SLN mapping, and 26 SLNs were identified in eight beagles. NIR imaging enabled high-spatial-resolution visualization of both SLNs and the intervening lymphatic vessels and was useful for intraoperative SLN navigation. CONCLUSION: SLN mapping with fluorescent iodized oil emulsion is effective and feasible for both CT and NIR imaging.
Full Text
http://pubs.rsna.org/doi/abs/10.1148/radiol.14141159
DOI
10.1148/radiol.14141159
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Kim, Hon Soul(김한솔)
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Lee, Sang Kil(이상길) ORCID logo https://orcid.org/0000-0002-0721-0364
Lim, Joon Seok(임준석) ORCID logo https://orcid.org/0000-0002-0334-5042
Hyung, Woo Jin(형우진) ORCID logo https://orcid.org/0000-0002-8593-9214
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139708
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