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Periodontitis mainly increases osteoclast formation via enhancing the differentiation of quiescent osteoclast precursors into osteoclasts

 D E Lee  ;  J H Kim  ;  S H Choi  ;  J H Cha  ;  E J Bak  ;  Y J Yoo 
 JOURNAL OF PERIODONTAL RESEARCH, Vol.50(2) : 256-264, 2015 
Journal Title
Issue Date
Alveolar Bone Loss/pathology ; Alveolar Process/pathology ; Animals ; Bromodeoxyuridine ; Cell Count ; Cell Differentiation/physiology ; Cell Nucleus/pathology ; Leukocyte Count ; Leukocytes, Mononuclear/pathology ; Male ; Neutrophils/pathology ; Osteoclasts/pathology ; Osteoclasts/physiology* ; Periodontitis/pathology* ; Rats ; Rats, Inbred F344 ; Stem Cells/physiology* ; Tartrate-Resistant Acid Phosphatase/analysis ; Time Factors ; Tooth Cervix/pathology
differentiation ; osteoclasts ; periodontitis ; quiescent osteoclast precursors
BACKGROUND AND OBJECTIVES: Tartrate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclasts are formed in sequential steps: proliferation and differentiation of hematopoietic progenitors into quiescent osteoclast precursors (QOPs), followed by fusion of QOPs. In this study, we investigated whether enhancement of osteoclast formation by periodontitis is derived from the stimulation of proliferation of hematopoietic progenitors or the differentiation of QOPs into osteoclasts. MATERIAL AND METHODS: Ligatures were placed around the first molars in the left mandibles of Fischer 344 inbred rats. The rats received drinking water containing bromodeoxyuridine (BrdU) (which can be incorporated into dividing nuclei) after ligation during the experimental period. The number of inflammatory cells in the distal area was counted. Alveolar bone loss was histologically estimated by measuring the distance from the cementoenamel junction to the alveolar bone crest in the distal area and determining the percentage of periodontal ligament area in the furcation. The number of osteoclasts and percentage of BrdU(+) nuclei in total osteoclasts nuclei were counted after TRAP and BrdU double labeling. RESULTS: The number of polymorphonuclear cells increased on day 1 and then rapidly decreased. The number of mononuclear cells increased in a time-dependent manner up to day 5 and remained the same until day 10. Alveolar bone loss of ligatured teeth increased in a time-dependent manner. The number of osteoclasts peaked on day 3 then gradually decreased. At peak, the percentage of BrdU(+) nuclei in total osteoclasts nuclei in the distal and furcation areas were 7.9% and 4.1%, respectively. CONCLUSION: These results indicate that most of the osteoclasts formed after periodontitis induction are derived from preformed QOPs, suggesting that enhancement of osteoclast formation by periodontitis might be mainly caused by stimulating the differentiation of QOPs into osteoclasts.
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Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
Yonsei Authors
Kim, Ji Hye(김지혜)
Bak, Eun-Jung(박은정) ORCID logo https://orcid.org/0000-0002-7976-8594
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Lee, Dong Eun(이동은)
Cha, Jung Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
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