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Diabetic characteristics and alveolar bone loss in streptozotocin- and streptozotocin-nicotinamide-treated rats with periodontitis.

Authors
 J H Kim  ;  D E Lee  ;  S H Choi  ;  J H Cha  ;  E J Bak  ;  Y J Yoo 
Citation
 JOURNAL OF PERIODONTAL RESEARCH, Vol.49(6) : 792-800, 2014 
Journal Title
 JOURNAL OF PERIODONTAL RESEARCH 
ISSN
 0022-3484 
Issue Date
2014
MeSH
Alveolar Bone Loss/etiology* ; Animals ; Blood Glucose/analysis ; Body Weight ; Bone Matrix/pathology ; Bone Resorption/etiology ; Diabetes Mellitus, Experimental/blood ; Diabetes Mellitus, Experimental/complications* ; Furcation Defects/etiology ; Gingiva/pathology ; Glucose Tolerance Test ; Hyperglycemia/blood ; Hyperglycemia/complications ; Insulin/blood ; Insulin-Secreting Cells/drug effects ; Leukocytes, Mononuclear/pathology ; Male ; Molar/pathology ; Neutrophils/pathology ; Niacinamide/administration & dosage* ; Periodontitis/complications* ; RANK Ligand/analysis ; Rats ; Rats, Inbred F344 ; Streptozocin ; Tibia/pathology ; Vitamin B Complex/administration & dosage* ; Weight Loss ; X-Ray Microtomography/methods
Keywords
bone loss ; nicotinamide ; periodontitis ; streptozotocin
Abstract
BACKGROUND AND OBJECTIVE: Experimental models showing variable diabetic status are necessary to understand the relationship between diabetes and periodontitis. The streptozotocin (STZ)-induced diabetes model allows control of diabetic status by nicotinamide (NA), which protects against STZ-induced β-cell necrosis. Therefore, we compared diabetic characteristics and alveolar bone loss in STZ- and STZ-NA-treated rats with periodontitis. MATERIAL AND METHODS: STZ-treated rats were generated by intravenous (IV) administration of STZ (50 mg/kg). STZ-NA-treated rats were induced by intraperitoneal administration of NA (270 mg/kg) 15 min before IV administration of STZ (65 mg/kg). Periodontitis was induced by ligature around the left mandibular first molar 1 wk after injection. Blood glucose level, glucose tolerance and serum insulin levels were determined at day 0 and/or 20 after ligature. At day 20, tibia bone loss was assessed using micro-computed tomography and hematoxylin and eosin staining. Alveolar bone loss was histologically measured as the distance of the cementoenamel junction to the alveolar bone crest in distal and the percentage of periodontal ligament area in the first molar furcation, respectively. The number of inflammatory cells, receptor activator of nuclear factor kappa-B ligand (RANKL)-positive cells and the area of osteoid were determined. RESULTS: In STZ-treated rats, obvious hyperglycemia over 300 mg/dL and severe body weight loss were observed. The insulin level was approximately 14% compared to that of control rats. STZ-NA-treated rats were impaired in glucose tolerance compared to control rats; however, body weight and insulin levels were not significantly different. Tibia bone loss was increased in STZ-treated rats, but significant change was not observed in STZ-NA-treated rats compared to control rats. In ligatured teeth, alveolar bone loss was increased in both STZ- and STZ-NA-treated rats compared to control rats. Alveolar bone loss, the number of inflammatory cells and RANKL-positive cells in STZ-treated rats were greater than in STZ-NA-treated rats. The area of osteoid decreased in STZ-treated rats compared to control, but not STZ-NA-treated rats. CONCLUSION: These results indicate that STZ- and STZ-NA-treated rats exhibit diabetic characteristics similar to type 1 diabetes mellitus and a pre-diabetic state, respectively. In addition, alveolar bone loss in response to periodontitis and tibia loss depend on diabetic status. Diabetic status-dependent bone remodeling imbalance and inflammation could affect the alveolar bone loss in the two models. Both STZ- and STZ-NA-treated rats may be useful to investigate differences in periodontitis sensitivity associated with diabetic status and to develop therapeutic agents for periodontitis in patients with diabetes.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/jre.12165/abstract
DOI
10.1111/jre.12165
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ji Hye(김지혜)
Bak, Eun-Jung(박은정) ORCID logo https://orcid.org/0000-0002-7976-8594
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Lee, Dong Eun(이동은)
Cha, Jung Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138636
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