1 238

Cited 24 times in

Thymidine phosphorylase suppresses apoptosis induced by microtubule-interfering agents

Authors
 Hei-Cheul Jeung  ;  Xiao-Fang Che  ;  Shin-ichi Akiyama  ;  Jae Kyung Roh  ;  Sun-Young Rha  ;  Tomoyuki Sumizawa  ;  Chun-Lei Zheng  ;  Tatsuhiko Furukawa  ;  Misako Haraguchi 
Citation
 BIOCHEMICAL PHARMACOLOGY, Vol.70(1) : 13-21, 2005 
Journal Title
 BIOCHEMICAL PHARMACOLOGY 
ISSN
 0006-2952 
Issue Date
2005
MeSH
Apoptosis/drug effects* ; Cell Line, Tumor ; Cytoprotection ; Fas Ligand Protein ; Humans ; Membrane Glycoproteins/analysis ; Membrane Glycoproteins/antagonists & inhibitors ; Microtubules/drug effects* ; Mitogen-Activated Protein Kinase 1/metabolism ; Mitogen-Activated Protein Kinase 3/metabolism ; Phosphorylation ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Thymidine Phosphorylase/physiology*
Keywords
Thymidine phosphorylase ; Microtubule ; Apoptosis ; Experimental therapeutics
Abstract
We investigated the ability of thymidine phosphorylase (TP) to confer cancer cells resistance to MIA (microtubule-interfering agents)-induced apoptosis. Jurkat cells were stably transfected with TP cDNA (Jurkat/TP) and the sensitivity to MIAs were examined. Jurkat/TP cells were more resistant to apoptosis induced by nocodazole, vincristine, vinblastine, paclitaxel and 2-methoxyestradiol than mock-trasfected Jurkat/CV cells. TP enzymatic activity was not required for this effect of TP. Jurkat/TP cells showed weak phosphorylation of Bcl-2, and kinase inhibitors staurosporine and genistein attenuated not only MIA-induced Bcl-2 phosphorylation but also cytotoxicity of MIA in Jurkat/CV, but not in Jurkat/TP. MIAs diminished expression of FasL in Jurkat/TP but not in Jurkat/CV, and neutralization of FasL by anti-FasL antibody considerably attenuated the cytotoxic effect of the MIAs in Jurkat/CV, but the effect of the antibody was marginal in Jurkat/TP cells. Our study provides further evidence that TP functions in conferring resistance on cancer cells to the stress induced by MIAs. In addition, we show that TP-induced inhibition of Bcl-2 phosphorylation and suppression of FasL may contribute to the protective function of TP in cancer cells.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006295205002017
DOI
10.1016/j.bcp.2005.04.017
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Cancer Metastasis Research Center (암전이연구센터) > 1. Journal Papers
Yonsei Authors
Roh, Jae Kyung(노재경)
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Jeung, Hei Cheul(정희철) ORCID logo https://orcid.org/0000-0003-0952-3679
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114794
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse