Cited 30 times in
Thymidine phosphorylase suppresses apoptosis induced by microtubule-interfering agents
DC Field | Value | Language |
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dc.contributor.author | 노재경 | - |
dc.contributor.author | 라선영 | - |
dc.contributor.author | 정희철 | - |
dc.date.accessioned | 2015-08-26T16:36:58Z | - |
dc.date.available | 2015-08-26T16:36:58Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0006-2952 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114794 | - |
dc.description.abstract | We investigated the ability of thymidine phosphorylase (TP) to confer cancer cells resistance to MIA (microtubule-interfering agents)-induced apoptosis. Jurkat cells were stably transfected with TP cDNA (Jurkat/TP) and the sensitivity to MIAs were examined. Jurkat/TP cells were more resistant to apoptosis induced by nocodazole, vincristine, vinblastine, paclitaxel and 2-methoxyestradiol than mock-trasfected Jurkat/CV cells. TP enzymatic activity was not required for this effect of TP. Jurkat/TP cells showed weak phosphorylation of Bcl-2, and kinase inhibitors staurosporine and genistein attenuated not only MIA-induced Bcl-2 phosphorylation but also cytotoxicity of MIA in Jurkat/CV, but not in Jurkat/TP. MIAs diminished expression of FasL in Jurkat/TP but not in Jurkat/CV, and neutralization of FasL by anti-FasL antibody considerably attenuated the cytotoxic effect of the MIAs in Jurkat/CV, but the effect of the antibody was marginal in Jurkat/TP cells. Our study provides further evidence that TP functions in conferring resistance on cancer cells to the stress induced by MIAs. In addition, we show that TP-induced inhibition of Bcl-2 phosphorylation and suppression of FasL may contribute to the protective function of TP in cancer cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 13~21 | - |
dc.relation.isPartOf | BIOCHEMICAL PHARMACOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis/drug effects* | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cytoprotection | - |
dc.subject.MESH | Fas Ligand Protein | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Membrane Glycoproteins/analysis | - |
dc.subject.MESH | Membrane Glycoproteins/antagonists & inhibitors | - |
dc.subject.MESH | Microtubules/drug effects* | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 1/metabolism | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 3/metabolism | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Proto-Oncogene Proteins c-bcl-2/metabolism | - |
dc.subject.MESH | Thymidine Phosphorylase/physiology* | - |
dc.title | Thymidine phosphorylase suppresses apoptosis induced by microtubule-interfering agents | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Hei-Cheul Jeung | - |
dc.contributor.googleauthor | Xiao-Fang Che | - |
dc.contributor.googleauthor | Shin-ichi Akiyama | - |
dc.contributor.googleauthor | Jae Kyung Roh | - |
dc.contributor.googleauthor | Sun-Young Rha | - |
dc.contributor.googleauthor | Tomoyuki Sumizawa | - |
dc.contributor.googleauthor | Chun-Lei Zheng | - |
dc.contributor.googleauthor | Tatsuhiko Furukawa | - |
dc.contributor.googleauthor | Misako Haraguchi | - |
dc.identifier.doi | 10.1016/j.bcp.2005.04.017 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01290 | - |
dc.contributor.localId | A01316 | - |
dc.contributor.localId | A03794 | - |
dc.relation.journalcode | J00283 | - |
dc.identifier.eissn | 1873-2968 | - |
dc.identifier.pmid | 15907805 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006295205002017 | - |
dc.subject.keyword | Thymidine phosphorylase | - |
dc.subject.keyword | Microtubule | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Experimental therapeutics | - |
dc.contributor.alternativeName | Roh, Jae Kyung | - |
dc.contributor.alternativeName | Rha, Sun Young | - |
dc.contributor.alternativeName | Jeung, Hei Cheul | - |
dc.contributor.affiliatedAuthor | Roh, Jae Kyung | - |
dc.contributor.affiliatedAuthor | Rha, Sun Young | - |
dc.contributor.affiliatedAuthor | Jeung, Hei Cheul | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 70 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 13 | - |
dc.citation.endPage | 21 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL PHARMACOLOGY, Vol.70(1) : 13-21, 2005 | - |
dc.identifier.rimsid | 38478 | - |
dc.type.rims | ART | - |
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