237 406

Cited 0 times in

Peroxisome Proliferator-Activated Receptor γ 활성을 통한 성인 중간엽 줄기 세포의 지방 세포 분화 촉진

Other Titles
 Peroxisome Proliferator-Activated Receptor γ Activation Promotes Adipogenesis in Human Mesenchymal Stem Cells 
Authors
 김은정  ;  김수향  ;  이진우  ;  한수봉  ;  김성재  ;  김윤희 
Citation
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.6(1) : 127-134, 2003 
Journal Title
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지) 
ISSN
 1226-8933 
Issue Date
2003
MeSH
Mesenchymal stem cell ; Adipocyte ; Peroxisome proliferator-activated receptor γ ; Troglitazone ; Mitogen activated protein kinase
Keywords
Mesenchymal stem cell ; Adipocyte ; Peroxisome proliferator-activated receptor γ ; Troglitazone ; Mitogen activated protein kinase
Abstract
Purpose: In this study, we determined that the troglitazones could induce uniform adipogenesis of human mesenchymal stem cells (MSCs) within a short time in a dose- and a time-dependent manners. Materials and Methods: Human MSCs were isolated from bone marrow and cultured in basal or adipogenic medium in the presence of 0 50 μM troglitazone for 5 days. Then we performed flow cytometry, RT-PCR and western blot analysis. Results: In FACS assay, troglitazone induced adipocyte differentiation in a dose-dependent manner. At concentration of 25 μM troglitazone in adipogenic medium, over 50% of the cells differentiated into adipocytes at day 5. This was accompanied by increased mRNA levels for the adipocyte gene markers (LPL, aP2 and PPARγ) in RT-PCR. In western blot analysis, we found that ERK phosphorylation was inhibited in the early stage of adipogenesis. Conclusion: Through the addition of troglitazone as a PPAR γ agonist, we could get the uniform adipogenic differentiation within a short time. Thus, troglitazone directly regulates differentiation of human MSCs into adipocytes; induced PPAR γ expression may play a key regulatory role in this process. And we suggest a role for ERK as a regulatory switch for these differentiation pathways
Files in This Item:
T200307017.pdf Download
DOI
OAK-2003-01452
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Kim, Sung Jae(김성재)
Kim, Su Hyang(김수향)
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
Hahn, Soo Bong(한수봉)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114612
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links