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Peroxisome Proliferator-Activated Receptor γ 활성을 통한 성인 중간엽 줄기 세포의 지방 세포 분화 촉진
DC Field | Value | Language |
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dc.contributor.author | 김성재 | - |
dc.contributor.author | 김수향 | - |
dc.contributor.author | 이진우 | - |
dc.contributor.author | 한수봉 | - |
dc.date.accessioned | 2015-07-15T17:17:25Z | - |
dc.date.available | 2015-07-15T17:17:25Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1226-8933 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114612 | - |
dc.description.abstract | Purpose: In this study, we determined that the troglitazones could induce uniform adipogenesis of human mesenchymal stem cells (MSCs) within a short time in a dose- and a time-dependent manners. Materials and Methods: Human MSCs were isolated from bone marrow and cultured in basal or adipogenic medium in the presence of 0 50 μM troglitazone for 5 days. Then we performed flow cytometry, RT-PCR and western blot analysis. Results: In FACS assay, troglitazone induced adipocyte differentiation in a dose-dependent manner. At concentration of 25 μM troglitazone in adipogenic medium, over 50% of the cells differentiated into adipocytes at day 5. This was accompanied by increased mRNA levels for the adipocyte gene markers (LPL, aP2 and PPARγ) in RT-PCR. In western blot analysis, we found that ERK phosphorylation was inhibited in the early stage of adipogenesis. Conclusion: Through the addition of troglitazone as a PPAR γ agonist, we could get the uniform adipogenic differentiation within a short time. Thus, troglitazone directly regulates differentiation of human MSCs into adipocytes; induced PPAR γ expression may play a key regulatory role in this process. And we suggest a role for ERK as a regulatory switch for these differentiation pathways | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Mesenchymal stem cell | - |
dc.subject.MESH | Adipocyte | - |
dc.subject.MESH | Peroxisome proliferator-activated receptor γ | - |
dc.subject.MESH | Troglitazone | - |
dc.subject.MESH | Mitogen activated protein kinase | - |
dc.title | Peroxisome Proliferator-Activated Receptor γ 활성을 통한 성인 중간엽 줄기 세포의 지방 세포 분화 촉진 | - |
dc.title.alternative | Peroxisome Proliferator-Activated Receptor γ Activation Promotes Adipogenesis in Human Mesenchymal Stem Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학) | - |
dc.contributor.googleauthor | 김은정 | - |
dc.contributor.googleauthor | 김수향 | - |
dc.contributor.googleauthor | 이진우 | - |
dc.contributor.googleauthor | 한수봉 | - |
dc.contributor.googleauthor | 김성재 | - |
dc.contributor.googleauthor | 김윤희 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00583 | - |
dc.contributor.localId | A00639 | - |
dc.contributor.localId | A03230 | - |
dc.contributor.localId | A04293 | - |
dc.relation.journalcode | J01526 | - |
dc.subject.keyword | Mesenchymal stem cell | - |
dc.subject.keyword | Adipocyte | - |
dc.subject.keyword | Peroxisome proliferator-activated receptor γ | - |
dc.subject.keyword | Troglitazone | - |
dc.subject.keyword | Mitogen activated protein kinase | - |
dc.contributor.alternativeName | Kim, Sung Jae | - |
dc.contributor.alternativeName | Kim, Su Hyang | - |
dc.contributor.alternativeName | Lee, Jin Woo | - |
dc.contributor.alternativeName | Hahn, Soo Bong | - |
dc.contributor.affiliatedAuthor | Kim, Sung Jae | - |
dc.contributor.affiliatedAuthor | Kim, Su Hyang | - |
dc.contributor.affiliatedAuthor | Lee, Jin Woo | - |
dc.contributor.affiliatedAuthor | Hahn, Soo Bong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 6 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 127 | - |
dc.citation.endPage | 134 | - |
dc.identifier.bibliographicCitation | Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.6(1) : 127-134, 2003 | - |
dc.identifier.rimsid | 40064 | - |
dc.type.rims | ART | - |
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