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치주조직 재생을 위한 칼슘 포스페이트 글라스가 MC3T3-E1 세포의 증식, 분화 및 석회화에 미치는 영향

Other Titles
 Influence of Calcium Phosphate Glass for Periodontal Regeneration on MC3T3-E1, Proliferation, Differentiation and Calcification 
 이용근  ;  송진  ;  최성호  ;  김경남  ;  이상배 
 Journal of the Korean Research Society for Dental Materials (대한치과기재학회지), Vol.30(2) : 181-188, 2003 
Journal Title
Journal of the Korean Research Society for Dental Materials(대한치과기재학회지)
Issue Date
bone graft ; calcium phosphate ; MC3T3-E1 ; cell ; proliferation ; differentiation ; calcification ; mineralization
bone graft ; calcium phosphate ; MC3T3-E1 ; cell ; proliferation ; differentiation ; calcification ; mineralization
The purpose of this work was to exploring the influence of the prepared calcium phosphate glass on the proliferation, differentiation, and mineralization of the culture system of the pre-osteoblastic MC3T3-E1 cells. Polystyrene was employed as a control in this experiment. Cells were cultured in a differentiated medium onto the calcium phosphate glass as well as polystyrene, and the number of cells and the protein contents were determined. To examine osteoblast differentiation, the alkaline phosphatase activity was measured. Mineralization was evaluated by staining the calcium deposit with Alizarin red. Culture onto the calcium phosphate glass exhibited no significant difference in cell proliferation compared with control group. The alkaline phosphatase activity in the experimental group, however, was enhanced by the calcium phosphate glass significantly at 10-18 days than that of the control group (p<0.05). Enhancement of bone-like tissue formation by the addition of the calcium phosphate glass was observed since 7 days. The results of the present study indicate that the prepared calcium phosphate glass promotes osteogenesis by increasing collagen synthesis and calcification of the extracellular matrix. It maybe due to greater calcium concentration in the culture medium released from the calcium phosphate glass.
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2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Yong Keun(이용근)
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
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