치주조직 재생을 위한 칼슘 포스페이트 글라스가 MC3T3-E1 세포의 증식, 분화 및 석회화에 미치는 영향

Abstract

The purpose of this work was to exploring the influence of the prepared calcium phosphate glass on the proliferation, differentiation, and mineralization of the culture system of the pre-osteoblastic MC3T3-E1 cells. Polystyrene was employed as a control in this experiment. Cells were cultured in a differentiated medium onto the calcium phosphate glass as well as polystyrene, and the number of cells and the protein contents were determined. To examine osteoblast differentiation, the alkaline phosphatase activity was measured. Mineralization was evaluated by staining the calcium deposit with Alizarin red. Culture onto the calcium phosphate glass exhibited no significant difference in cell proliferation compared with control group. The alkaline phosphatase activity in the experimental group, however, was enhanced by the calcium phosphate glass significantly at 10-18 days than that of the control group (p<0.05). Enhancement of bone-like tissue formation by the addition of the calcium phosphate glass was observed since 7 days. The results of the present study indicate that the prepared calcium phosphate glass promotes osteogenesis by increasing collagen synthesis and calcification of the extracellular matrix. It maybe due to greater calcium concentration in the culture medium released from the calcium phosphate glass.