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Liposomal Entrapment of Cefoxitin to Improve Cellular Viability and Function in Human Saphenous Veins

 Jong-Chul Park  ;  Hwal Suh  ;  Bum Koo Cho  ;  Young Hwan Park  ;  Bong Joo Park  ;  Dong Hee Lee  ;  Dong-Wook Han  ;  Hak-Joon Sung 
 ARTIFICIAL ORGANS, Vol.27(7) : 623-630, 2003 
Journal Title
Issue Date
Anti-Bacterial Agents/administration & dosage* ; Anti-Bacterial Agents/pharmacokinetics ; Bacteria/drug effects ; Cefoxitin/administration & dosage* ; Cefoxitin/pharmacokinetics ; Cell Survival/drug effects ; Coronary Artery Bypass ; Drug Carriers ; Endothelium, Vascular/cytology ; Female ; Humans ; In Vitro Techniques ; Liposomes ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Nitric Oxide Synthase/analysis ; Nitric Oxide Synthase Type III ; Saphenous Vein/cytology* ; Saphenous Vein/metabolism ; Saphenous Vein/microbiology ; Saphenous Vein/transplantation ; Sterilization
Liposomal cefoxitin ; Human saphenous vein ; Cellular viability ; Endothelial nitric oxide synthase ; Sterilization effect
Liposomal cefoxitin was prepared and applied to the pretreatment of human saphenous vein (HSV) for implantation. The possible use of liposomal cefoxitin to improve cellular viability and function and to maintain its potential sterilization effect was investigated. Entrapment efficiency and size distribution of liposomal cefoxitin were 75.7% and 652 ± 75.7 nm, respectively. The weight ratio between cefoxitin and liposome was calculated at 1 : 40.6. When cefoxitin was entrapped with liposome, the released amount of cefoxitin was not affected by temperature conditions (37°C, 25°C, and 4°C). The amount of free cefoxitin present in HSV reached 59% at 0.5 h and gradually decreased with time, while liposomal cefoxitin showed a maximum amount (63%) at 1.5 h, indicating that liposomal cefoxitin seemed to control the initial amount of cefoxitin present in HSV. Liposomal cefoxitin showed better viabilities of whole cells and endothelial cells dissociated from HSV than free cefoxitin and remarkably superior function of endothelial cells, as determined by Griffonia simplicifolia agglutinins-fluorescein isothiocyanate/propidium iodide double-staining methods combined with flow cytometry and endothelial nitric oxide synthase assay, respectively. In terms of sterilization effect, there was no significant difference between liposomal cefoxitin and free cefoxitin. These results suggest that liposomal entrapment of cefoxitin could improve cellular viability and functions and maintain the original sterilization effect.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Park, Bong Joo(박봉주)
Park, Young Hwan(박영환)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Suh, Hwal(서활)
Sung, Hak-Joon(성학준) ORCID logo https://orcid.org/0000-0003-2312-2484
Cho, Bum Koo(조범구)
Han, Dong Wook(한동욱)
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