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The Effects of Recombinant Human BMP-7, Prepared from a COS-7 Expression System, on the Proliferation and Differentiation of Rat Newborn Calvarial Osteoblasts

Authors
 Dong Hee Lee  ;  Hwal Suh  ;  Jong Chul Park  ;  Jin Woo Lee  ;  Bong Joo Park  ;  Dong Wook Han 
Citation
 YONSEI MEDICAL JOURNAL, Vol.44(4) : 593-601, 2003 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
2003
MeSH
Bone morphogenetic protein ; COS-7 ; rat newborn calvaria cell ; osteoblast ; DNA synthesis ; alkaline phosphatase ; cAMP ; osteocalcin
Keywords
Bone morphogenetic protein ; COS-7 ; rat newborn calvaria cell ; osteoblast ; DNA synthesis ; alkaline phosphatase ; cAMP ; osteocalcin
Abstract
A family of proteins, the bone morphogenetic proteins (BMPs), which promote osteoblast differentiation and bone mineralization, have recently been identified. One, BMP-7, has shown the ability to induce cartilage and bone formation processes. In this report, the possibility that other cell lines, to CHO cells, may also be available as host cells for the expression of hBMP-7 was validated. Recombinant human BMP (rhBMP)-7 was produced in COS-7 cells, as a processed mature disulfide-linked homodimer, with an apparent molecular weight of 36, 000. Examination of the expressions of the markers characteristic of osteoblast phenotypes showed that the rhBMP-7 specifically stimulated the inductions of alkaline phosphatase (ALP) (5-fold increase at 100 ng of rhBMP-7/ml), parathyroid hormone (PTH)-mediated intracellular cAMP production (4-fold increase at 100 ng of rhBMP-7/ml) and osteocalcin synthesis (5-fold increase at 100 ng of rhBMP-7/ml). In summary, the in vitro mineralization assay results provide evidence that the rhBMP-7 peptide, produced by COS-7 expression system, possesses intact biological activity. A similar pattern of biological activity was observed for the BMP-7 in COS-7 cells compared to the corresponding CHO cell expression system. Thus, these findings can be experimentally utilized for the production of rhBMPs for in vitro or in vivo studies.
Files in This Item:
T200302684.pdf Download
DOI
OAK-2003-00190
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Bong Joo(박봉주)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Suh, Hwal(서활)
Lee, Dong Hee(이동희)
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
Han, Dong Wook(한동욱)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/113350
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