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The Effects of Recombinant Human BMP-7, Prepared from a COS-7 Expression System, on the Proliferation and Differentiation of Rat Newborn Calvarial Osteoblasts

DC FieldValueLanguage
dc.contributor.author박봉주-
dc.contributor.author박종철-
dc.contributor.author서활-
dc.contributor.author이동희-
dc.contributor.author이진우-
dc.contributor.author한동욱-
dc.date.accessioned2015-07-15T16:39:53Z-
dc.date.available2015-07-15T16:39:53Z-
dc.date.issued2003-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/113350-
dc.description.abstractA family of proteins, the bone morphogenetic proteins (BMPs), which promote osteoblast differentiation and bone mineralization, have recently been identified. One, BMP-7, has shown the ability to induce cartilage and bone formation processes. In this report, the possibility that other cell lines, to CHO cells, may also be available as host cells for the expression of hBMP-7 was validated. Recombinant human BMP (rhBMP)-7 was produced in COS-7 cells, as a processed mature disulfide-linked homodimer, with an apparent molecular weight of 36, 000. Examination of the expressions of the markers characteristic of osteoblast phenotypes showed that the rhBMP-7 specifically stimulated the inductions of alkaline phosphatase (ALP) (5-fold increase at 100 ng of rhBMP-7/ml), parathyroid hormone (PTH)-mediated intracellular cAMP production (4-fold increase at 100 ng of rhBMP-7/ml) and osteocalcin synthesis (5-fold increase at 100 ng of rhBMP-7/ml). In summary, the in vitro mineralization assay results provide evidence that the rhBMP-7 peptide, produced by COS-7 expression system, possesses intact biological activity. A similar pattern of biological activity was observed for the BMP-7 in COS-7 cells compared to the corresponding CHO cell expression system. Thus, these findings can be experimentally utilized for the production of rhBMPs for in vitro or in vivo studies.-
dc.description.statementOfResponsibilityopen-
dc.format.extent593~601-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBone morphogenetic protein-
dc.subject.MESHCOS-7-
dc.subject.MESHrat newborn calvaria cell-
dc.subject.MESHosteoblast-
dc.subject.MESHDNA synthesis-
dc.subject.MESHalkaline phosphatase-
dc.subject.MESHcAMP-
dc.subject.MESHosteocalcin-
dc.titleThe Effects of Recombinant Human BMP-7, Prepared from a COS-7 Expression System, on the Proliferation and Differentiation of Rat Newborn Calvarial Osteoblasts-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentMedical Research Center (임상의학연구센터)-
dc.contributor.googleauthorDong Hee Lee-
dc.contributor.googleauthorHwal Suh-
dc.contributor.googleauthorJong Chul Park-
dc.contributor.googleauthorJin Woo Lee-
dc.contributor.googleauthorBong Joo Park-
dc.contributor.googleauthorDong Wook Han-
dc.identifier.doiOAK-2003-00190-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01482-
dc.contributor.localIdA01662-
dc.contributor.localIdA01924-
dc.contributor.localIdA03230-
dc.contributor.localIdA04275-
dc.contributor.localIdA02743-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.subject.keywordBone morphogenetic protein-
dc.subject.keywordCOS-7-
dc.subject.keywordrat newborn calvaria cell-
dc.subject.keywordosteoblast-
dc.subject.keywordDNA synthesis-
dc.subject.keywordalkaline phosphatase-
dc.subject.keywordcAMP-
dc.subject.keywordosteocalcin-
dc.contributor.alternativeNamePark, Bong Joo-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.alternativeNameSuh, Hwal-
dc.contributor.alternativeNameLee, Dong Hee-
dc.contributor.alternativeNameLee, Jin Woo-
dc.contributor.alternativeNameHan, Dong Wook-
dc.contributor.affiliatedAuthorPark, Bong Joo-
dc.contributor.affiliatedAuthorPark, Jong Chul-
dc.contributor.affiliatedAuthorSuh, Hwal-
dc.contributor.affiliatedAuthorLee, Jin Woo-
dc.contributor.affiliatedAuthorHan, Dong Wook-
dc.contributor.affiliatedAuthorLee, Dong Hee-
dc.rights.accessRightsfree-
dc.citation.volume44-
dc.citation.number4-
dc.citation.startPage593-
dc.citation.endPage601-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.44(4) : 593-601, 2003-
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers

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