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Two novel mutations of Wiskott - Aldrich syndrome: The molecular prediction of interaction between the mutated WASP L101P with WASP-interacting protein by molecular modeling

Authors
 Moon Kyu Kim  ;  Eun Sook Kim  ;  Jeon-Soo Shin  ;  Chang No Yoon  ;  Taesung Moon  ;  In-Hong Choi  ;  Dong Soo Kim 
Citation
 BIOCHIMICA ET BIOPHYSICA ACTA , Vol.1690(2) : 134-140, 2004 
Journal Title
 BIOCHIMICA ET BIOPHYSICA ACTA 
ISSN
 0006-3002 
Issue Date
2004
MeSH
Amino Acid Sequence ; Base Sequence ; Carrier Proteins/chemistry* ; Carrier Proteins/genetics* ; Cell Division ; Chromosomes, Human, X ; Cytoskeletal Proteins ; DNA Mutational Analysis ; Exons ; Female ; Frameshift Mutation ; Gene Deletion ; Humans ; Hydrogen Bonding ; Inosine Triphosphate/genetics ; Intracellular Signaling Peptides and Proteins ; Magnetic Resonance Spectroscopy ; Male ; Models, Molecular ; Molecular Sequence Data ; Mutation* ; Mutation, Missense ; Pedigree ; Peptides/chemistry ; Protein Binding ; Protein Conformation ; Protein Structure, Tertiary ; Proteins/chemistry* ; Proteins/genetics* ; Sequence Homology, Amino Acid ; T-Lymphocytes/metabolism ; Wiskott-Aldrich Syndrome/genetics* ; Wiskott-Aldrich Syndrome Protein
Keywords
Wiskott–Aldrich syndrome (WAS) ; WAS protein (WASP) ; WASP-interacting protein (WIP) ; Molecular modeling
Abstract
Wiskott–Aldrich syndrome (WAS) is an X-linked disorder characterized by eczema, thrombocytopenia and increased susceptibility of infections, with mutations of the WAS gene being responsible for WAS and X-linked thrombocytopenia. Herein, two novel mutations of WAS at T336C on exon 3, and at 1326–1329, a G deletion on exon 10, resulting in L101P missense mutation and frameshift mutation 444 stop, respectively, are reported. The affected patients with either mutation showed severe suppression of WAS protein (WASP) levels, T cell proliferation, and CFSE-labeled T cells division. Because WASP L101 have not shown direct nuclear Overhauser effect (NOE) contact with the WASP-interacting protein (WIP) in NMR spectroscopy, molecular modeling was performed to evaluate the molecular effect of WASP P101 to WIP peptide. It is presumed that P101 induced a conformational change in the Q99 residue of WASP and made the side chain of Q99 move away from the WIP peptide, resulting in disruption of the hydrogen bond between Q99 WASP and Y475 WIP. A possible model for the molecular pathogenesis of WAS has been proposed by analyzing the interactions of WASP and WIP using a molecular modeling study.
Files in This Item:
T200401435.pdf Download
DOI
10.1016/j.bbadis.2004.06.007
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Soo(김동수)
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/112576
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