The human myotubularin-related protein suppresses the growth of lung carcinoma cells

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Authors: Young Do Yoo ; Sun Mi Cho ; Hyung Jung Kim ; Chul Min Ahn ; Yoon Soo Chang ; Jun Suk Kim

MeSH: Blotting, Northern ; Cell Cycle ; Cell Cycle Proteins/metabolism ; Cell Differentiation ; Cell Division ; Cell Line, Tumor ; Cell Separation ; Cyclin-Dependent Kinase Inhibitor p27 ; DNA/chemistry ; DNA, Complementary/metabolism ; Dose-Response Relationship, Drug ; Flow Cytometry ; G1 Phase ; Genetic Therapy/methods* ; Genetic Vectors ; Humans ; Immunoblotting ; Lung Neoplasms/metabolism* ; Lung Neoplasms/therapy* ; Protein Tyrosine Phosphatases/genetics ; Protein Tyrosine Phosphatases/physiology* ; Protein Tyrosine Phosphatases, Non-Receptor ; Transfection ; Treatment Outcome ; Tumor Suppressor Proteins/metabolism

Abstract: Herein, we report that the human myotubularin-related protein (KIAA0371) induced growth suppression of lung cancer cells. Colony formation assays demonstrated that the exogenous expression of the human myotubularin-related gene induced a significant growth inhibition of H1299 cells in which the expression of the human myotubularin-related gene was undetectable. Colony formation of these cells was reduced by 39% after transfection with this gene, as compared with the control vector. Cells stably transfected with this gene showed a reduction in growth of 48% compared with those cells transfected with the empty vector. We also showed that this negative regulation of the myotubularin-related protein is correlated with cell-cycle arrest at G1. In addition, we found that the cyclin-dependent kinase inhibitor, p27, is induced by increased expression of the human myotubularin-related gene. These results suggest that the human myotubularin-related protein can negatively regulate the growth of lung cancer cells.