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Expression of a novel secreted splice variant of the receptor for advanced glycation end products (RAGE) in human brain astrocytes and peripheral blood mononuclear cells

 In Ho Park  ;  Soo In Yeon  ;  Jeon-Soo Shin  ;  In-Hong Choi  ;  Nobuyuki Sasaki  ;  Ji Eun Choi  ;  Ju Ho Youn 
 MOLECULAR IMMUNOLOGY, Vol.40(16) : 1203-1211, 2004 
Journal Title
Issue Date
Alternative Splicing* ; Amino Acid Sequence ; Astrocytes/metabolism* ; Brain/cytology ; Codon, Terminator ; Exons ; Frameshift Mutation ; Gene Expression ; Glycation End Products, Advanced/chemistry ; Glycation End Products, Advanced/genetics ; Glycation End Products, Advanced/metabolism* ; Humans ; Leukocytes, Mononuclear/metabolism* ; Molecular Sequence Data ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Receptors, Immunologic/genetics* ; Receptors, Immunologic/metabolism ; Recombinant Proteins/immunology ; Recombinant Proteins/metabolism ; Sequence Homology, Amino Acid
The engagement of the receptor for advanced glycation end products (RAGE) on the cell surface induces cellular dysfunction in a number of pathophysiological situations of vascular dysfunction, tumor cell invasion, inflammatory response, and T cell infiltration. The administration of truncated, soluble RAGE can modulate RAGE-mediated perturbations. Here, we report a novel splice variant (Δ8-RAGE) of RAGE mRNA, which lacks exon 8 of the genomic RAGE gene and contains an early stop codon in exon 10 due to a frame shift mutation. Δ8-RAGE mRNA was found in human primary astrocytes and peripheral blood mononuclear cells (PBMCs). Transient transfection experiments demonstrated that Δ8-RAGE mRNA was translated into a secretory protein as deduced. Moreover, two different segments of the spliced variant were identified in PBMCs by RT-PCR. The findings of this study suggest that the diverse splicing variants of RAGE are possible in many tissues and their products may influence the RAGE-mediated pathogenesis and immune modulation.
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1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Jeon Soo(신전수) ORCID logo https://orcid.org/0000-0002-8294-3234
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
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