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Analysis of gene expression profiles of hepatocellular carcinomas with regard to 18F-fluorodeoxyglucose uptake pattern on positron emission tomography

 Jong Doo Lee  ;  Mijin Yun  ;  Jeon Han Park  ;  Sang Moo Lim  ;  Naechun Yoo  ;  Woo Jung Lee  ;  Kwang-Hyub Han  ;  Young Nyun Park  ;  Woo Ick Yang  ;  Kyung Sik Kim  ;  Se Jong Kim  ;  Ji Su Kim  ;  Youn-Hee Choi  ;  Youjeong Choi  ;  Jae Myun Lee 
 European Journal of Nuclear Medicine and Molecular Imaging, Vol.31(12) : 1621-1630, 2004 
Journal Title
 European Journal of Nuclear Medicine and Molecular Imaging 
Issue Date
Adult ; Aged ; Biomarkers, Tumor/metabolism* ; Carcinoma, Hepatocellular/diagnostic imaging* ; Carcinoma, Hepatocellular/genetics ; Carcinoma, Hepatocellular/metabolism* ; Female ; Fluorodeoxyglucose F18/pharmacokinetics* ; Gene Expression Profiling/methods ; Gene Expression Regulation, Neoplastic ; Humans ; Liver Neoplasms/diagnostic imaging* ; Liver Neoplasms/genetics ; Liver Neoplasms/metabolism* ; Male ; Middle Aged ; Neoplasm Proteins/metabolism* ; Oligonucleotide Array Sequence Analysis/methods ; Organ Specificity ; Radionuclide Imaging ; Radiopharmaceuticals/pharmacokinetics ; Reproducibility of Results ; Sensitivity and Specificity ; Severity of Illness Index ; Statistics as Topic ; Tissue Distribution
Hepatocellular carcinoma ; F-FDG uptake ; Positron emission tomography ; Gene expression profile ; OligoDNA microarray
PURPOSE: 18F-fluorodeoxyglucose (FDG) uptake on positron emission tomography (PET) scan has been found to reflect tumour aggressiveness and prognosis in various types of cancer. In this study, the gene expression profiles of hepatocellular carcinomas (HCCs) were evaluated to determine whether HCCs with high 18F-FDG uptake have more aggressive biological potential than those with low uptake. METHODS: Surgical specimens were obtained from ten patients with HCC (six males and four females, age range 38-68 years). The tumour samples were divided into two groups based on the 18F-FDG PET scan findings: high 18F-FDG uptake (n=4) and low 18F-FDG uptake (n=6). RESULTS: The pathological tumour grade was closely correlated with the 18F-FDG uptake pattern: HCCs with high 18F-FDG uptake were pathologically Edmondson-Steiner grade III, while those with low uptake were either grade II or grade II with a focal area of grade III. The total RNA was extracted from the frozen tissues of all HCCs (n=10) and adjacent non-cancerous tissue (n=7). The gene expression profiles were evaluated using an oligoDNA microarray. The HCCs with high 18F-FDG uptake showed increased expression of 11 genes--including vascular cell adhesion molecule-1, vinexin beta and core 1 UDP-galactose:N-acetylgalactosamine-alpha-R-beta 1,3-galactosyltransferase and the natural killer cell inhibitory receptor--compared to those with low uptake (p<0.005). Nine genes, including regulator of mitotic spindle assembly 1, grb2-related adaptor protein and beta-1,3-n-acetylglucosaminyltransferase, were repressed. CONCLUSION: Gene expression is closely related to cell survival, cell-to-cell adhesion or cell spreading; therefore, HCCs with high 18F-FDG uptake appear to have more aggressive biological properties than those with low uptake.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sik(김경식) ORCID logo https://orcid.org/0000-0001-9498-284X
Kim, Se Jong(김세종)
Kim, Ji Su(김지수)
Park, Young Nyun(박영년) ORCID logo https://orcid.org/0000-0003-0357-7967
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Yoo, Nae Choon(유내춘)
Yun, Mi Jin(윤미진) ORCID logo https://orcid.org/0000-0002-1712-163X
Lee, Woo Jung(이우정) ORCID logo https://orcid.org/0000-0001-9273-261X
Lee, Jae Myun(이재면) ORCID logo https://orcid.org/0000-0002-5273-3113
Lee, Jong Doo(이종두)
Choi, Youn Hee(최윤희)
Han, Kwang-Hyub(한광협) ORCID logo https://orcid.org/0000-0003-3960-6539
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