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Short-term chemical disinfection 후 가타파차 콘의 멸균 효과와 표면 성상 및 물성 변화의 평가

Other Titles
 Assessment of sterilization effect and the alteration of surface texture and physical properties of gutta-percha cone after short-term chemical disinfection 
Authors
 Nan-Sim Pang  ;  Il-Young Jung  ;  Yoon-Jung Yu  ;  Kee-Yeon Kum 
Citation
 Journal of Korean Academy of Operative Dentistry (대한치과보존학회지), Vol.31(2) : 133-140, 2006 
Journal Title
 Journal of Korean Academy of Operative Dentistry (대한치과보존학회지) 
ISSN
 1225-0864 
Issue Date
2006
Keywords
PCR ; Surface texture ; Physical properties ; Sterilization effect ; Gutta-percha cone
Abstract
The purposes of this study were firstly to identify the microbial species on gutta-percha (GP) cones exposed at clinics using polymerase chain reaction, and secondly to evaluate the short-term sterilization effect of three chemical disinfectants. It also evaluated the alteration of surface texture and physical properties of GP cones after 5-min soaking into three chemical disinfectants. 150 GP cones from two endodontic departments were randomly selected for microbial detection using PCR assay with universal primer. After inoculation on the sterilized GP cones with the same microorganism identified by PCR assay, they were soaked in three chemical disinfectants: 5% NaOCl, 2% Chlorhexidine, and ChloraPrep for 1, 5, 10, and 30 minutes. The sterilization effect was evaluated by turbidity and subculture. The change of surface textures using a scanning electron microscope and the tensile strength and elongation rate of the GP cones were measured using an Instron 5500 (Canton). Statistical analysis was performed. Four bacterial species were detected in 29 GP cones (19.4%), and all the species belonged to the genus Staphylococcus. All chemical disinfectants were effective in sterilization with just 1 minute soaking. On the SEM picture of NaOCl-soaked GP cone, a cluster of cuboidal crystals was seen on the cone surface. The tensile strength of NaOCl-soaked group was significantly higher than the other groups (p < 0.05). Also, all disinfectants significantly increased the elongation rate of GP cones compared to the fresh GP cone (p < 0.05). Present data demonstrate that three chemical disinfectants are useful for rapid sterilization of GP cone just before obturation.
Files in This Item:
T200601184.pdf Download
DOI
10.5395/JKACD.2006.31.2.133
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Advanced General Dentistry (통합치의학과) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
Yonsei Authors
Pang, Nan Sim(방난심) ORCID logo https://orcid.org/0000-0003-4265-673X
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Jung, Il Young(정일영) ORCID logo https://orcid.org/0000-0001-8972-2664
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110159
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