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Minocycline inhibits caspase-dependent and -independent cell death pathways and is neuroprotective against hippocampal damage after treatment with kainic acid in mice

 Kyoung Heo  ;  Yang-Je Cho  ;  Kyoung-Joo Cho  ;  Hyun-Woo Kim  ;  Hyun-Jung Kim  ;  Ha Young Shin  ;  Byung In Lee  ;  Gyung Whan Kim 
 NEUROSCIENCE LETTERS, Vol.398(3) : 195-200, 2006 
Journal Title
Issue Date
Animals ; Apoptosis/physiology* ; Apoptosis Inducing Factor/metabolism ; Caspase 3 ; Caspases/metabolism ; Caspases/physiology* ; Cytochromes c/metabolism ; Disease Models, Animal ; Epilepsy, Temporal Lobe/chemically induced ; Epilepsy, Temporal Lobe/pathology ; Epilepsy, Temporal Lobe/prevention & control* ; Hippocampus/drug effects* ; Hippocampus/metabolism ; Hippocampus/pathology ; Kainic Acid* ; Male ; Mice ; Mice, Inbred ICR ; Minocycline/pharmacology* ; Neuroprotective Agents/pharmacology*
Minocycline ; Neuroprotection ; Kainic acid ; Hippocampus ; Apoptosis
Although minocycline has been generally thought to have neuroprotective properties, the neuroprotective role of minocycline has not been investigated in the animal model of epilepsy. In this study, we investigated whether minocycline is neuroprotective against kainic acid (KA)-induced cell death through the caspase-dependent or -independent mitochondrial apoptotic pathways. Adult male ICR mice were subjected to seizures by intrahippocampal KA injection with vehicle or with minocycline. For cell death analysis, TdT-mediated dUTP-biotin nick end labeling and cresyl-violet staining were performed. Western blot analysis and immunofluorescent staining for cytochrome c and apoptosis-inducing factor (AIF) were performed. Cell death was reduced in minocycline-treated mice. Cytosolic translocation of cytochrome c and subsequent activation of caspase-3 were diminished by minocycline treatment. AIF nuclear translocation and subsequent large-scale DNA fragmentation were also reduced in minocycline-treated mice. Thus, this study suggests that minocycline inhibits both caspase-dependent and -independent apoptotic pathways and may be neuroprotective against hippocampal damage after KA treatment.
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1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Gyung Whan(김경환) ORCID logo https://orcid.org/0000-0001-7053-4372
Kim, Hyun Woo(김현우)
Kim, Hyun Jeong(김현정)
Shin, Ha Young(신하영) ORCID logo https://orcid.org/0000-0002-4408-8265
Lee, Byung In(이병인)
Cho, Kyuong Joo(조경주)
Cho, Yang Je(조양제)
Heo, Kyoung(허경)
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