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Shank2 Associates with and Regulates Na+/H+ Exchanger 3

Authors
 WonSun Han  ;  Kyung Hwan Kim  ;  Min Jae Jo  ;  Ji Hyun Lee  ;  Jinhee Yang  ;  R. Brian Doctor  ;  Orson W. Moe  ;  Jinu Lee  ;  Eunjoon Kim  ;  Min Goo Lee 
Citation
 JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.281(3) : 1461-1469, 2006 
Journal Title
 JOURNAL OF BIOLOGICAL CHEMISTRY 
ISSN
 0021-9258 
Issue Date
2006
MeSH
Animals ; Cell Line ; Cell Line, Tumor ; Colonic Neoplasms ; Cricetinae ; Gene Deletion ; Genes, Reporter ; Humans ; Kinetics ; Molecular Sequence Data ; Nerve Tissue Proteins/chemistry ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism* ; Sodium-Hydrogen Exchangers/chemistry ; Sodium-Hydrogen Exchangers/genetics ; Sodium-Hydrogen Exchangers/metabolism* ; Surface Plasmon Resonance ; Transfection
Abstract
Na+/H+ exchanger 3 (NHE3) plays a pivotal role in transepithelial Na+ and Formula absorption across a wide range of epithelia in the digestive and renal-genitourinary systems. Accumulating evidence suggests that PDZ-based adaptor proteins play an important role in regulating the trafficking and activity of NHE3. A search for NHE3-binding modular proteins using yeast two-hybrid assays led us to the PDZ-based adaptor Shank2. The interaction between Shank2 and NHE3 was further confirmed by immunoprecipitation and surface plasmon resonance studies. When expressed in PS120/NHE3 cells, Shank2 increased the membrane expression and basal activity of NHE3 and attenuated the cAMP-dependent inhibition of NHE3 activity. Furthermore, knock-down of native Shank2 expression in Caco-2 epithelial cells by RNA interference decreased NHE3 protein expression as well as activity but amplified the inhibitory effect of cAMP on NHE3. These results indicate that Shank2 is a novel NHE3 interacting protein that is involved in the fine regulation of transepithelial salt and water transport through affecting NHE3 expression and activity.
Files in This Item:
T200600035.pdf Download
DOI
10.1074/jbc.M509786200
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
Lee, Min Goo(이민구) ORCID logo https://orcid.org/0000-0001-7436-012X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108784
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