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Shank2 Associates with and Regulates Na+/H+ Exchanger 3

DC Field Value Language
dc.contributor.author김경환-
dc.contributor.author이민구-
dc.date.accessioned2015-06-10T11:52:42Z-
dc.date.available2015-06-10T11:52:42Z-
dc.date.issued2006-
dc.identifier.issn0021-9258-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/108784-
dc.description.abstractNa+/H+ exchanger 3 (NHE3) plays a pivotal role in transepithelial Na+ and Formula absorption across a wide range of epithelia in the digestive and renal-genitourinary systems. Accumulating evidence suggests that PDZ-based adaptor proteins play an important role in regulating the trafficking and activity of NHE3. A search for NHE3-binding modular proteins using yeast two-hybrid assays led us to the PDZ-based adaptor Shank2. The interaction between Shank2 and NHE3 was further confirmed by immunoprecipitation and surface plasmon resonance studies. When expressed in PS120/NHE3 cells, Shank2 increased the membrane expression and basal activity of NHE3 and attenuated the cAMP-dependent inhibition of NHE3 activity. Furthermore, knock-down of native Shank2 expression in Caco-2 epithelial cells by RNA interference decreased NHE3 protein expression as well as activity but amplified the inhibitory effect of cAMP on NHE3. These results indicate that Shank2 is a novel NHE3 interacting protein that is involved in the fine regulation of transepithelial salt and water transport through affecting NHE3 expression and activity.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1461~1469-
dc.relation.isPartOfJOURNAL OF BIOLOGICAL CHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Line-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHColonic Neoplasms-
dc.subject.MESHCricetinae-
dc.subject.MESHGene Deletion-
dc.subject.MESHGenes, Reporter-
dc.subject.MESHHumans-
dc.subject.MESHKinetics-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHNerve Tissue Proteins/chemistry-
dc.subject.MESHNerve Tissue Proteins/genetics-
dc.subject.MESHNerve Tissue Proteins/metabolism*-
dc.subject.MESHSodium-Hydrogen Exchangers/chemistry-
dc.subject.MESHSodium-Hydrogen Exchangers/genetics-
dc.subject.MESHSodium-Hydrogen Exchangers/metabolism*-
dc.subject.MESHSurface Plasmon Resonance-
dc.subject.MESHTransfection-
dc.titleShank2 Associates with and Regulates Na+/H+ Exchanger 3-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorWonSun Han-
dc.contributor.googleauthorKyung Hwan Kim-
dc.contributor.googleauthorMin Jae Jo-
dc.contributor.googleauthorJi Hyun Lee-
dc.contributor.googleauthorJinhee Yang-
dc.contributor.googleauthorR. Brian Doctor-
dc.contributor.googleauthorOrson W. Moe-
dc.contributor.googleauthorJinu Lee-
dc.contributor.googleauthorEunjoon Kim-
dc.contributor.googleauthorMin Goo Lee-
dc.identifier.doi10.1074/jbc.M509786200-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00311-
dc.contributor.localIdA02781-
dc.relation.journalcodeJ01258-
dc.identifier.eissn1083-351X-
dc.identifier.pmid16293618-
dc.contributor.alternativeNameKim, Kyung Hwan-
dc.contributor.alternativeNameLee, Min Goo-
dc.contributor.affiliatedAuthorKim, Kyung Hwan-
dc.contributor.affiliatedAuthorLee, Min Goo-
dc.rights.accessRightsfree-
dc.citation.volume281-
dc.citation.number3-
dc.citation.startPage1461-
dc.citation.endPage1469-
dc.identifier.bibliographicCitationJOURNAL OF BIOLOGICAL CHEMISTRY, Vol.281(3) : 1461-1469, 2006-
dc.identifier.rimsid57699-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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