Cited 48 times in
Shank2 Associates with and Regulates Na+/H+ Exchanger 3
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.contributor.author | 이민구 | - |
dc.date.accessioned | 2015-06-10T11:52:42Z | - |
dc.date.available | 2015-06-10T11:52:42Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108784 | - |
dc.description.abstract | Na+/H+ exchanger 3 (NHE3) plays a pivotal role in transepithelial Na+ and Formula absorption across a wide range of epithelia in the digestive and renal-genitourinary systems. Accumulating evidence suggests that PDZ-based adaptor proteins play an important role in regulating the trafficking and activity of NHE3. A search for NHE3-binding modular proteins using yeast two-hybrid assays led us to the PDZ-based adaptor Shank2. The interaction between Shank2 and NHE3 was further confirmed by immunoprecipitation and surface plasmon resonance studies. When expressed in PS120/NHE3 cells, Shank2 increased the membrane expression and basal activity of NHE3 and attenuated the cAMP-dependent inhibition of NHE3 activity. Furthermore, knock-down of native Shank2 expression in Caco-2 epithelial cells by RNA interference decreased NHE3 protein expression as well as activity but amplified the inhibitory effect of cAMP on NHE3. These results indicate that Shank2 is a novel NHE3 interacting protein that is involved in the fine regulation of transepithelial salt and water transport through affecting NHE3 expression and activity. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1461~1469 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Colonic Neoplasms | - |
dc.subject.MESH | Cricetinae | - |
dc.subject.MESH | Gene Deletion | - |
dc.subject.MESH | Genes, Reporter | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Kinetics | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Nerve Tissue Proteins/chemistry | - |
dc.subject.MESH | Nerve Tissue Proteins/genetics | - |
dc.subject.MESH | Nerve Tissue Proteins/metabolism* | - |
dc.subject.MESH | Sodium-Hydrogen Exchangers/chemistry | - |
dc.subject.MESH | Sodium-Hydrogen Exchangers/genetics | - |
dc.subject.MESH | Sodium-Hydrogen Exchangers/metabolism* | - |
dc.subject.MESH | Surface Plasmon Resonance | - |
dc.subject.MESH | Transfection | - |
dc.title | Shank2 Associates with and Regulates Na+/H+ Exchanger 3 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | WonSun Han | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.contributor.googleauthor | Min Jae Jo | - |
dc.contributor.googleauthor | Ji Hyun Lee | - |
dc.contributor.googleauthor | Jinhee Yang | - |
dc.contributor.googleauthor | R. Brian Doctor | - |
dc.contributor.googleauthor | Orson W. Moe | - |
dc.contributor.googleauthor | Jinu Lee | - |
dc.contributor.googleauthor | Eunjoon Kim | - |
dc.contributor.googleauthor | Min Goo Lee | - |
dc.identifier.doi | 10.1074/jbc.M509786200 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00311 | - |
dc.contributor.localId | A02781 | - |
dc.relation.journalcode | J01258 | - |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.pmid | 16293618 | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 281 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1461 | - |
dc.citation.endPage | 1469 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.281(3) : 1461-1469, 2006 | - |
dc.identifier.rimsid | 57699 | - |
dc.type.rims | ART | - |
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