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일과성 국소 대뇌 허혈 생쥐 모델에서 APE/Ref-1 융합 아데노바이러스 처치에 의한 APE/Ref-1의 과발현 및 신경세포고사 감소

Other Titles
 Overexpressed Apurinic/Apyrimidinic Endonuclease/Redox Effector Factor-1 Using Adenoviral Vector Inhibits Induction of Neuronal Cell Death after Transient Ischemic Stroke in Mice 
Authors
 김현우  ;  조경주  ;  이병인  ;  김현정  ;  김경환 
Citation
 Journal of Neurocritical Care, Vol.1(2) : 150-156, 2008 
Journal Title
Journal of Neurocritical Care
ISSN
 2005-0348 
Issue Date
2008
Keywords
Cerebral ischemia and/or reperfusion ; DNA damage and repair ; Stroke treatment ; Adenoviral vectors
Abstract
Background: Despite the correlation between changes in the levels of apurinic/apyrimidinic endonuclease/redox effector factor-1 (APE/Ref-1), the DNA base excision repair protein, and ischemic neuronal damage, no studies have addressed the question of whether the overexpression or restoration of APE/Ref-1 may prevent ischemic neuronal cell death in vivo. Therefore, we investigated whether increasing APE/Ref-1 may inhibit the loss of APE/Ref-1 and DNA fragmentation, and prevent neuronal cell death after cerebral ischemia/reperfusion (I/R). Methods: Adult male C57BL/6J mice were subjected to intraluminal suture occlusion of the middle cerebral artery for 1 hour followed by reperfusion. Pre-ischemic treatment of the adenoviral vector harboring an entire APE/Ref-1 gene sequence was introduced intrastriatally. Immunohistochemistry assays in AP sites, and TUNEL were performed. Results: APE/Ref-1 was overexpressed by using adenoviral-vector-mediated APE/Ref-1 in striatum. Pre-ischemic administration of the Adenoviral vector harboring an APE/Ref-1 gene, by 4 or 24 hours after reperfusion, significantly decreased AP site and 8-OHdG, and subsequent inhibited the induction of apoptotic DNA fragmentation at 24 hours after I/R. Conclusion: The overexpression of APE/Ref-1 can prevent induction of apoptotic cell death after I/R. Using adenoviral-vector-mediated APE/Ref-1 could represent a molecular target for prevention of ischemic neuronal cell death.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Gyung Whan(김경환) ORCID logo https://orcid.org/0000-0001-7053-4372
Kim, Hyun Woo(김현우)
Kim, Hyun Jeong(김현정)
Lee, Byung In(이병인)
Cho, Kyuong Joo(조경주)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108374
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