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일과성 국소 대뇌 허혈 생쥐 모델에서 APE/Ref-1 융합 아데노바이러스 처치에 의한 APE/Ref-1의 과발현 및 신경세포고사 감소
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.contributor.author | 김현우 | - |
dc.contributor.author | 김현정 | - |
dc.contributor.author | 이병인 | - |
dc.contributor.author | 조경주 | - |
dc.date.accessioned | 2015-05-19T17:33:33Z | - |
dc.date.available | 2015-05-19T17:33:33Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 2005-0348 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108374 | - |
dc.description.abstract | Background: Despite the correlation between changes in the levels of apurinic/apyrimidinic endonuclease/redox effector factor-1 (APE/Ref-1), the DNA base excision repair protein, and ischemic neuronal damage, no studies have addressed the question of whether the overexpression or restoration of APE/Ref-1 may prevent ischemic neuronal cell death in vivo. Therefore, we investigated whether increasing APE/Ref-1 may inhibit the loss of APE/Ref-1 and DNA fragmentation, and prevent neuronal cell death after cerebral ischemia/reperfusion (I/R). Methods: Adult male C57BL/6J mice were subjected to intraluminal suture occlusion of the middle cerebral artery for 1 hour followed by reperfusion. Pre-ischemic treatment of the adenoviral vector harboring an entire APE/Ref-1 gene sequence was introduced intrastriatally. Immunohistochemistry assays in AP sites, and TUNEL were performed. Results: APE/Ref-1 was overexpressed by using adenoviral-vector-mediated APE/Ref-1 in striatum. Pre-ischemic administration of the Adenoviral vector harboring an APE/Ref-1 gene, by 4 or 24 hours after reperfusion, significantly decreased AP site and 8-OHdG, and subsequent inhibited the induction of apoptotic DNA fragmentation at 24 hours after I/R. Conclusion: The overexpression of APE/Ref-1 can prevent induction of apoptotic cell death after I/R. Using adenoviral-vector-mediated APE/Ref-1 could represent a molecular target for prevention of ischemic neuronal cell death. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 150~156 | - |
dc.relation.isPartOf | Journal of Neurocritical Care | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 일과성 국소 대뇌 허혈 생쥐 모델에서 APE/Ref-1 융합 아데노바이러스 처치에 의한 APE/Ref-1의 과발현 및 신경세포고사 감소 | - |
dc.title.alternative | Overexpressed Apurinic/Apyrimidinic Endonuclease/Redox Effector Factor-1 Using Adenoviral Vector Inhibits Induction of Neuronal Cell Death after Transient Ischemic Stroke in Mice | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurology (신경과학) | - |
dc.contributor.googleauthor | 김현우 | - |
dc.contributor.googleauthor | 조경주 | - |
dc.contributor.googleauthor | 이병인 | - |
dc.contributor.googleauthor | 김현정 | - |
dc.contributor.googleauthor | 김경환 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00310 | - |
dc.contributor.localId | A02797 | - |
dc.contributor.localId | A03804 | - |
dc.contributor.localId | A01125 | - |
dc.contributor.localId | A01130 | - |
dc.relation.journalcode | J01621 | - |
dc.identifier.pmid | Cerebral ischemia and/or reperfusion; DNA damage and repair ; Stroke treatment ; Adenoviral vectors | - |
dc.subject.keyword | Cerebral ischemia and/or reperfusion | - |
dc.subject.keyword | DNA damage and repair | - |
dc.subject.keyword | Stroke treatment | - |
dc.subject.keyword | Adenoviral vectors | - |
dc.contributor.alternativeName | Kim, Gyung Whan | - |
dc.contributor.alternativeName | Kim, Hyun Woo | - |
dc.contributor.alternativeName | Kim, Hyun Jeong | - |
dc.contributor.alternativeName | Lee, Byung In | - |
dc.contributor.alternativeName | Cho, Kyuong Joo | - |
dc.contributor.affiliatedAuthor | Kim, Gyung Whan | - |
dc.contributor.affiliatedAuthor | Lee, Byung In | - |
dc.contributor.affiliatedAuthor | Cho, Kyuong Joo | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Woo | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Jeong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 1 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 150 | - |
dc.citation.endPage | 156 | - |
dc.identifier.bibliographicCitation | Journal of Neurocritical Care, Vol.1(2) : 150-156, 2008 | - |
dc.identifier.rimsid | 35526 | - |
dc.type.rims | ART | - |
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