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Morphological evidence of basal keratinocyte migration during the re-epithelialization process

Authors
 Akihiro Hosoya  ;  Jong-Min Lee  ;  Sung-Won Cho  ;  Ji-Youn Kim  ;  Naoshi Shinozaki  ;  Takahiko Shibahara  ;  Masaki Shimono  ;  Han-Sung Jung 
Citation
 HISTOCHEMISTRY AND CELL BIOLOGY, Vol.130(6) : 1165-1175, 2008 
Journal Title
HISTOCHEMISTRY AND CELL BIOLOGY
ISSN
 0948-6143 
Issue Date
2008
MeSH
Animals ; Animals, Newborn ; Bromodeoxyuridine/metabolism ; Cell Differentiation* ; Cell Movement* ; Epithelial Cells/metabolism ; Epithelial Cells/pathology* ; Epithelial Cells/radiation effects ; Keratin-14/metabolism ; Keratin-15 ; Keratin-5/metabolism ; Keratinocytes/metabolism ; Keratinocytes/pathology* ; Keratinocytes/radiation effects ; Lasers, Gas ; Mice ; Mice, Inbred ICR ; Mouth Mucosa/metabolism ; Mouth Mucosa/pathology* ; Mouth Mucosa/radiation effects ; Phosphoproteins/metabolism ; Proliferating Cell Nuclear Antigen/metabolism ; Stem Cells/metabolism ; Stem Cells/pathology* ; Stem Cells/radiation effects ; Time Factors ; Trans-Activators/metabolism ; Wound Healing*
Keywords
Re-epithelialization ; Basal keratinocyte ; Epithelial stem cell ; BrdU labeling ; Oral mucosa
Abstract
The regeneration of wounded stratified epithelium is accomplished via the migration of keratinocytes from the margins of the wound. However, the process of keratinocyte migration on the wound surface and the role of epithelial stem cells during re-epithelialization remain to be elucidated. Therefore, we administered BrdU to embryonic mice and generated epithelial defects on the buccal mucosa of these mice at two weeks after birth, using CO(2) laser irradiation, with which we removed the entire thickness of the epithelium. In the unwounded epithelium, cytokeratin 14, p63, and BrdU were localized within the basal layer of the epithelium, but the majority of cells within the regenerated epithelium were immunopositive for these proteins. PCNA-negative and BrdU-positive basal keratinocytes, which evidence a slow cell cycle, were localized solely within the basal layer of the unwound epithelium facing the tips of dermal papillae. After laser irradiation, these basal keratinocytes facing the tips of the papillae evidenced positive immunoreactivity for PCNA, in addition to BrdU. These results indicate that epithelial stem cells of oral mucosa may be localized in the basal layer of the epithelium facing the tips of dermal papillae, and may migrate laterally with other basal keratinocytes in response to external stimuli
Full Text
http://link.springer.com/article/10.1007%2Fs00418-008-0499-3
DOI
10.1007/s00418-008-0499-3
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108069
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