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자기장 저속 냉동보관법을 이용한 쥐 치아 치주인대세포의 활성도 검사

Other Titles
 Evaluation of the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field 
Authors
 안현정  ;  김의성  ;  김 진  ;  김덕원  ;  김기열  ;  이찬영  ;  이승종 
Citation
 Journal of Korean Academy of Operative Dentistry (대한치과보존학회지), Vol.33(4) : 332-340, 2008 
Journal Title
 Journal of Korean Academy of Operative Dentistry (대한치과보존학회지) 
ISSN
 1225-0864 
Issue Date
2008
Keywords
TUNEL ; Periodontal ligament cell ; MTT ; Magnetic field ; Cryopreservation
Abstract
The purpose of this study was to evaluate the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field through MTT assay and TUNEL test. For each group, 12 teeth of 4 weeks old white female Sprague-Dawley rat were used for MTT assay, and 6 teeth in TUNEL test. The Maxillary left and right, first and second molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group1 (immediately extraction), group 2 (cold preservation at 4°C for 1 week), group 3 (rapid cryopreservation in liquid nitrogen), group 4 (slow cryopreservation with magnetic field of 1 G), and group 5 (slow cryopreservation). F medium was used as preservation medium and 10% DMSO as cryoprotectant. After preservation and thawing, the MTT assay and TUNEL test were processed. One way ANOVA and Scheffe method were performed at the 95% level of confidence. The value of optical density obtained after MTT analysis was divided by the value of eosin staining for tissue volume standardization. In both MTT assay and TUNEL test, it had showed no significant difference among group 3, 4, and 5. And group 3 had showed higher viability of periodontal ligament cell than group 2. From this study, slow cryopreservation method with magnetic field can be used as one of cryopreservation methods
Files in This Item:
T200803119.pdf Download
DOI
10.5395/JKACD.2008.33.4.332
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ki Yeol(김기열) ORCID logo https://orcid.org/0000-0001-5357-1067
Kim, Deok Won(김덕원)
Kim, Eui Seong(김의성) ORCID logo https://orcid.org/0000-0003-2126-4761
Kim, Jin(김진)
Lee, Seung Jong(이승종)
Lee, Chan Young(이찬영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108045
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