자발 운동 및 부유 환경 조성에 의한 선조체 내 신경세포 생성 유도

Abstract

Objective: To investigate that neurogenesis in the subventricular zone (SVZ), which is already known as neurogenic area where neural stem/progenitor cells persist, and the striatum, which is non-neurogenic area, might be induced by voluntary exercise (VEx) or environmental enrichment (EE), and compare the extent of the neurogenesis with untreated controls. Method: Total 12 C57BL/6 mice, 2∼3 months old, were recruited as follows; voluntary wheel runner, EE and control. For 2 weeks, VEx group was housed in rat cage (48×26 cm) with 2 running wheels with 3∼4 animals/cage, and EE group was housed in the living condition of huge cage (86×76 cm), social interaction (13∼14 mice/cage) and objects such as toys, tunnels and running wheel, whereas control group was placed in the standard cage (30×18 cm). Results: VEx and EE tended to increase the densities of mitotic marker BrdU＋ cells in SVZ and striatum. They also exhibited more BrdU＋ cells (/mm3) into the striatum, even though they did not show statistical significance. Moreover, EE group showed significant increment of the newly generated neurons coexpressed with BrdU＋ and ՂIII-tubulin＋ (/mm3) in SVZ and striatum as compared to those of controls. Conclusion: Voluntary physical exercise and EE induced cell proliferation and neurogenesis in both SVZ and striatum. Characteristically, EE could significantly induce neurogenesis in striatum, non-neurogenic area as well as SVZ, typical neurogenic area. Therefore, this strategy might be used to activate neural regeneration in various central nervous system diseases