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Induction of IL-8 in periodontal ligament cells by H(2)O (2).

Authors
 Yang-Sin Lee  ;  Eun Jung Bak  ;  Minyoung Kim  ;  Wonse Park  ;  Jeong Taeg Seo  ;  Yun-Jung Yoo 
Citation
 JOURNAL OF MICROBIOLOGY, Vol.46(5) : 579-584, 2008 
Journal Title
JOURNAL OF MICROBIOLOGY
ISSN
 1225-8873 
Issue Date
2008
MeSH
Cell Survival/drug effects ; Cells, Cultured ; Gene Expression/drug effects ; Humans ; Hydrogen Peroxide/pharmacology* ; Interleukin-8/genetics ; Interleukin-8/immunology* ; Mitogen-Activated Protein Kinases/genetics ; Mitogen-Activated Protein Kinases/metabolism ; Periodontal Ligament/drug effects* ; Periodontal Ligament/immunology* ; Periodontitis/immunology* ; Periodontitis/microbiology ; Phosphorylation ; Signal Transduction/drug effects ; Up-Regulation/drug effects*
Keywords
IL-8 ; periodontal ligament cells ; H2O2
Abstract
Periodontitis is an inflammatory disease caused by bacteria. In periodontitis, reactive oxygen species (ROS) are released from inflammatory cells in response to bacteria. Interleukin (IL)-8 is one of pro-inflammatory cytokines. To investigate the role of ROS in pathogenesis of periodontitis, we estimated the effect of H(2)O(2), one of ROS, on the expression of IL-8 in human periodontal ligament (PDL) cells. PDL cells were treated with H(2)O(2). IL-8 expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38) and c-jun NH(2)-terminal kinase (JNK) was estimated by Western blotting. Treatment with H(2)O(2) at concentration of up to 250 microM increased IL-8 mRNA expression and production in a concentration-dependent manner. However, treatment with 500 microM H(2)O(2) did not increase IL-8 production. Catalase, an inhibitor of H(2)O(2), down-regulated the production of IL-8 induced by H(2)O(2). H(2)O(2) increased the phosphorylation of ERK, p38, and JNK. Pretreatment with PD98059 (ERK inhibitor), SB203580 (p38 inhibitor), or SP600125 (JNK inhibitor) decreased the IL-8 production induced by H(2)O(2). These results indicate that H(2)O(2) acts as an inducer of IL-8 secretion via activation of ERK, p38, and JNK in PDL cells. H(2)O(2) deposited in periodontal tissue during inflammation against bacteria may accelerate tissue destruction via induction of IL-8 in PDL cells.
Full Text
http://link.springer.com/article/10.1007%2Fs12275-008-0182-3
DOI
10.1007/s12275-008-0182-3
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Advanced General Dentistry (통합치의학과) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Min Young(김민영)
Park, Wonse(박원서) ORCID logo https://orcid.org/0000-0002-2081-1156
Seo, Jeong Taeg(서정택) ORCID logo https://orcid.org/0000-0003-2697-0251
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Lee, Yang Sin(이양신)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107766
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