Cited 19 times in
Induction of IL-8 in periodontal ligament cells by H(2)O (2).
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김민영 | - |
dc.contributor.author | 박원서 | - |
dc.contributor.author | 서정택 | - |
dc.contributor.author | 유윤정 | - |
dc.contributor.author | 이양신 | - |
dc.date.accessioned | 2015-05-19T17:14:19Z | - |
dc.date.available | 2015-05-19T17:14:19Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/107766 | - |
dc.description.abstract | Periodontitis is an inflammatory disease caused by bacteria. In periodontitis, reactive oxygen species (ROS) are released from inflammatory cells in response to bacteria. Interleukin (IL)-8 is one of pro-inflammatory cytokines. To investigate the role of ROS in pathogenesis of periodontitis, we estimated the effect of H(2)O(2), one of ROS, on the expression of IL-8 in human periodontal ligament (PDL) cells. PDL cells were treated with H(2)O(2). IL-8 expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38) and c-jun NH(2)-terminal kinase (JNK) was estimated by Western blotting. Treatment with H(2)O(2) at concentration of up to 250 microM increased IL-8 mRNA expression and production in a concentration-dependent manner. However, treatment with 500 microM H(2)O(2) did not increase IL-8 production. Catalase, an inhibitor of H(2)O(2), down-regulated the production of IL-8 induced by H(2)O(2). H(2)O(2) increased the phosphorylation of ERK, p38, and JNK. Pretreatment with PD98059 (ERK inhibitor), SB203580 (p38 inhibitor), or SP600125 (JNK inhibitor) decreased the IL-8 production induced by H(2)O(2). These results indicate that H(2)O(2) acts as an inducer of IL-8 secretion via activation of ERK, p38, and JNK in PDL cells. H(2)O(2) deposited in periodontal tissue during inflammation against bacteria may accelerate tissue destruction via induction of IL-8 in PDL cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 579~584 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Gene Expression/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hydrogen Peroxide/pharmacology* | - |
dc.subject.MESH | Interleukin-8/genetics | - |
dc.subject.MESH | Interleukin-8/immunology* | - |
dc.subject.MESH | Mitogen-Activated Protein Kinases/genetics | - |
dc.subject.MESH | Mitogen-Activated Protein Kinases/metabolism | - |
dc.subject.MESH | Periodontal Ligament/drug effects* | - |
dc.subject.MESH | Periodontal Ligament/immunology* | - |
dc.subject.MESH | Periodontitis/immunology* | - |
dc.subject.MESH | Periodontitis/microbiology | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Up-Regulation/drug effects* | - |
dc.title | Induction of IL-8 in periodontal ligament cells by H(2)O (2). | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of General Dentistry (통합진료학) | - |
dc.contributor.googleauthor | Yang-Sin Lee | - |
dc.contributor.googleauthor | Eun Jung Bak | - |
dc.contributor.googleauthor | Minyoung Kim | - |
dc.contributor.googleauthor | Wonse Park | - |
dc.contributor.googleauthor | Jeong Taeg Seo | - |
dc.contributor.googleauthor | Yun-Jung Yoo | - |
dc.identifier.doi | 10.1007/s12275-008-0182-3 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01589 | - |
dc.contributor.localId | A01905 | - |
dc.contributor.localId | A02490 | - |
dc.contributor.localId | A02948 | - |
dc.contributor.localId | A00465 | - |
dc.relation.journalcode | J01593 | - |
dc.identifier.eissn | 1976-3794 | - |
dc.identifier.pmid | 18974961 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs12275-008-0182-3 | - |
dc.subject.keyword | IL-8 | - |
dc.subject.keyword | periodontal ligament cells | - |
dc.subject.keyword | H2O2 | - |
dc.contributor.alternativeName | Kim, Min Young | - |
dc.contributor.alternativeName | Park, Wonse | - |
dc.contributor.alternativeName | Seo, Jeong Taeg | - |
dc.contributor.alternativeName | Yoo, Yun Jung | - |
dc.contributor.alternativeName | Lee, Yang Sin | - |
dc.contributor.affiliatedAuthor | Park, Wonse | - |
dc.contributor.affiliatedAuthor | Seo, Jeong Taeg | - |
dc.contributor.affiliatedAuthor | Yoo, Yun Jung | - |
dc.contributor.affiliatedAuthor | Lee, Yang Sin | - |
dc.contributor.affiliatedAuthor | Kim, Min Young | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 46 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 579 | - |
dc.citation.endPage | 584 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, Vol.46(5) : 579-584, 2008 | - |
dc.identifier.rimsid | 34669 | - |
dc.type.rims | ART | - |
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