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Imaging of the inflammatory response in reperfusion injury after transient cerebral ischemia in rats: correlation of superparamagnetic iron oxide-enhanced magnetic resonance imaging with histopathology.

 Kim J  ;  Kim D  ;  Lee SK  ;  Kim DJ  ;  Lee JE  ;  Ahn SK. 
 ACTA RADIOLOGICA, Vol.49(5) : 580-588, 2008 
Journal Title
Issue Date
Animals ; Brain/pathology ; Brain/ultrastructure ; Contrast Media/administration & dosage ; Disease Models, Animal ; Ferrosoferric Oxide* ; Image Enhancement/methods* ; Imaging, Three-Dimensional ; Inflammation/diagnosis* ; Inflammation/etiology ; Ischemic Attack, Transient/complications ; Ischemic Attack, Transient/diagnosis* ; Macrophages/pathology ; Macrophages/ultrastructure ; Magnetic Resonance Imaging/methods* ; Male ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury/diagnosis* ; Reperfusion Injury/etiology ; Time Factors
Acute ischemic stroke ; contrast media ; ferucarbotran ; inflammation ; macrophage ; magnetic resonance imaging ; reperfusion
BACKGROUND: Acute inflammatory responses have been thought to play a central role in ischemia-reperfusion injury after acute ischemic stroke. Superparamagnetic iron oxide (SPIO) particles have been known to enable in-vivo monitoring of macrophage infiltration by magnetic resonance imaging (MRI) in the experimental ischemic rat brain. PURPOSE: To determine whether the accumulation of macrophages could be seen in vivo in a reperfusion animal model after focal cerebral ischemia using SPIO-enhanced MRI. MATERIAL AND METHODS: Thirty-four adult male rats were enrolled in this study. SPIO particles were injected into the rats at different time points after 1-hour transient occlusion of the middle cerebral artery, and three-dimensional (3D) T2*-weighted magnetic resonance (MR) images with a gradient-echo sequence were performed 24 hours later. Histochemical iron staining was compared with T2* signal abnormalities. RESULTS: At days 3 and 4 post-reperfusion, focal areas of signal loss indicating local accumulation of SPIO particles appeared in a part of the damaged brain. Areas of signal loss corresponded to local accumulation of iron-laden macrophages in histologic sections, and SPIO-induced signal loss indicated active macrophage transmigration into the reperfused brain. CONCLUSION: SPIO-enhanced MRI demonstrated through in-vivo monitoring that macrophages participate in reperfusion injury at early stages of injury development. SPIO-enhanced MRI could be a useful tool to examine the inflammatory mechanisms involved in reperfusion brain injury.
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1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Ik(김동익)
Kim, Dong Joon(김동준) ORCID logo https://orcid.org/0000-0002-7035-087X
Kim, Jinna(김진아) ORCID logo https://orcid.org/0000-0002-9978-4356
Lee, Seung Koo(이승구) ORCID logo https://orcid.org/0000-0001-5646-4072
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