1 246

Cited 34 times in

Therapeutic effect of magnesium lithospermate B on neointimal formation after balloon-induced vascular injury

Authors
 Kyu Yeon Hur  ;  Hye Jun Seo  ;  Eun Seok Kang  ;  Soo Hyun Kim  ;  Seungjeong Song  ;  Eun Hee Kim  ;  Soyeon Lim  ;  Chulhee Choi  ;  Ji Hoe Heo  ;  Ki Chul Hwang  ;  Chul Woo Ahn  ;  Bong Soo Cha  ;  Mankil Jung  ;  Hyun Chul Lee 
Citation
 European Journal of Pharmacology, Vol.586(1-3) : 226-233, 2008 
Journal Title
 European Journal of Pharmacology 
ISSN
 0014-2999 
Issue Date
2008
Abstract
Vascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor (PDGF) play an important role in the development of atherosclerosis and restenosis. Recent evidence indicates that PDGF increases intracellular levels of reactive oxygen species in VSMCs and that both PDGF-induced VSMC proliferation and migration are reactive oxygen species-dependent. Danshen is a representative oriental medicine used for the treatment of vascular disease. Previously, we reported that magnesium lithospermate B, an active component of Danshen, is a potent antioxidant. Thus we investigated the therapeutic potential of magnesium lithospermate B in neointimal formation after carotid artery injury in rats along with its effects on the PDGF signaling pathway for stimulating VSMC proliferation and migration in vitro. PDGF is dimeric glycoprotein composed of two A or two B chains. In this study, we used PDGF-BB, which is one of the isoforms of PDGF (i.e., PDGF-AA, PDGF-BB, and PDGF-AB). Our results demonstrated that magnesium lithospermate B directly scavenged reactive oxygen species in a xanthine/xanthine oxidase system and reduced PDGF-BB-induced intracellular reactive oxygen species generation in VSMCs. In a rat carotid artery balloon injury model, magnesium lithospermate B treatment (10 mg/kg/day, i.p) showed a significant effect on the prevention of neointimal formation compared with vehicle treatment. In cultured VSMCs, magnesium lithospermate B significantly attenuated PDGF-BB-induced cell proliferation and migration as measured by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2-tetrazolium bromide (MTT) assay and transwell migration assays, respectively. Further, magnesium lithospermate B inhibited PDGF-BB-induced phosphorylation of phospatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathways by scavenging reactive oxygen species. Together, these data indicated that magnesium lithospermate B, a potent reactive oxygen species scavenger, prevented both injury-induced neointimal formation in vivo and PDGF-BB-induced VSMC proliferation and migration in vitro, suggesting that magnesium lithospermate B may be a promising agent to prevent atherosclerosis and restenosis following angioplasty.
Full Text
http://www.sciencedirect.com/science/article/pii/S0014299908001714
DOI
10.1016/j.ejphar.2008.02.072
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
강은석(Kang, Eun Seok) ORCID logo https://orcid.org/0000-0002-0364-4675
김수현(Kim, Soo Hyun)
김은희(Kim, Eun Hee)
서혜선(Seo, Hye Sun)
안철우(Ahn, Chul Woo) ORCID logo https://orcid.org/0000-0003-3733-7486
이현철(Lee, Hyun Chul)
임소연(Lim, So Yeon)
차봉수(Cha, Bong Soo) ORCID logo https://orcid.org/0000-0003-0542-2854
허규연(Hur, Kyu Yeon)
허지회(Heo, Ji Hoe) ORCID logo https://orcid.org/0000-0001-9898-3321
황기철(Hwang, Ki Chul)
Export
RIS (EndNote)
XLS (Excel)
XML
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106593
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse