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Therapeutic effect of magnesium lithospermate B on neointimal formation after balloon-induced vascular injury

DC FieldValueLanguage
dc.contributor.author강은석-
dc.contributor.author김수현-
dc.contributor.author김은희-
dc.contributor.author서혜선-
dc.contributor.author안철우-
dc.contributor.author이현철-
dc.contributor.author임소연-
dc.contributor.author차봉수-
dc.contributor.author허규연-
dc.contributor.author허지회-
dc.contributor.author황기철-
dc.date.accessioned2015-05-19T16:35:44Z-
dc.date.available2015-05-19T16:35:44Z-
dc.date.issued2008-
dc.identifier.issn0014-2999-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/106593-
dc.description.abstractVascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor (PDGF) play an important role in the development of atherosclerosis and restenosis. Recent evidence indicates that PDGF increases intracellular levels of reactive oxygen species in VSMCs and that both PDGF-induced VSMC proliferation and migration are reactive oxygen species-dependent. Danshen is a representative oriental medicine used for the treatment of vascular disease. Previously, we reported that magnesium lithospermate B, an active component of Danshen, is a potent antioxidant. Thus we investigated the therapeutic potential of magnesium lithospermate B in neointimal formation after carotid artery injury in rats along with its effects on the PDGF signaling pathway for stimulating VSMC proliferation and migration in vitro. PDGF is dimeric glycoprotein composed of two A or two B chains. In this study, we used PDGF-BB, which is one of the isoforms of PDGF (i.e., PDGF-AA, PDGF-BB, and PDGF-AB). Our results demonstrated that magnesium lithospermate B directly scavenged reactive oxygen species in a xanthine/xanthine oxidase system and reduced PDGF-BB-induced intracellular reactive oxygen species generation in VSMCs. In a rat carotid artery balloon injury model, magnesium lithospermate B treatment (10 mg/kg/day, i.p) showed a significant effect on the prevention of neointimal formation compared with vehicle treatment. In cultured VSMCs, magnesium lithospermate B significantly attenuated PDGF-BB-induced cell proliferation and migration as measured by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2-tetrazolium bromide (MTT) assay and transwell migration assays, respectively. Further, magnesium lithospermate B inhibited PDGF-BB-induced phosphorylation of phospatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathways by scavenging reactive oxygen species. Together, these data indicated that magnesium lithospermate B, a potent reactive oxygen species scavenger, prevented both injury-induced neointimal formation in vivo and PDGF-BB-induced VSMC proliferation and migration in vitro, suggesting that magnesium lithospermate B may be a promising agent to prevent atherosclerosis and restenosis following angioplasty.-
dc.description.statementOfResponsibilityopen-
dc.format.extent226~233-
dc.relation.isPartOfEUROPEAN JOURNAL OF PHARMACOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAngiogenesis Inhibitors/therapeutic use*-
dc.subject.MESHAnimals-
dc.subject.MESHAntioxidants/therapeutic use*-
dc.subject.MESHAorta, Thoracic/cytology-
dc.subject.MESHAorta, Thoracic/drug effects-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCarotid Artery Injuries/drug therapy*-
dc.subject.MESHCarotid Artery Injuries/pathology*-
dc.subject.MESHCatheterization-
dc.subject.MESHCell Movement/drug effects-
dc.subject.MESHCell Proliferation/drug effects-
dc.subject.MESHCell Separation-
dc.subject.MESHDrugs, Chinese Herbal/isolation & purification-
dc.subject.MESHDrugs, Chinese Herbal/therapeutic use*-
dc.subject.MESHElectrophoresis, Polyacrylamide Gel-
dc.subject.MESHFree Radical Scavengers/therapeutic use*-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHMale-
dc.subject.MESHMyocytes, Smooth Muscle/drug effects-
dc.subject.MESHNeovascularization, Pathologic/drug therapy*-
dc.subject.MESHPlant Roots/chemistry-
dc.subject.MESHPlatelet-Derived Growth Factor/antagonists & inhibitors-
dc.subject.MESHPlatelet-Derived Growth Factor/pharmacology-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHReactive Oxygen Species/metabolism-
dc.subject.MESHSalvia miltiorrhiza/chemistry-
dc.subject.MESHSignal Transduction/drug effects-
dc.titleTherapeutic effect of magnesium lithospermate B on neointimal formation after balloon-induced vascular injury-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentMedical Research Center (임상의학연구센터)-
dc.contributor.googleauthorKyu Yeon Hur-
dc.contributor.googleauthorHye Jun Seo-
dc.contributor.googleauthorEun Seok Kang-
dc.contributor.googleauthorSoo Hyun Kim-
dc.contributor.googleauthorSeungjeong Song-
dc.contributor.googleauthorEun Hee Kim-
dc.contributor.googleauthorSoyeon Lim-
dc.contributor.googleauthorChulhee Choi-
dc.contributor.googleauthorJi Hoe Heo-
dc.contributor.googleauthorKi Chul Hwang-
dc.contributor.googleauthorChul Woo Ahn-
dc.contributor.googleauthorBong Soo Cha-
dc.contributor.googleauthorMankil Jung-
dc.contributor.googleauthorHyun Chul Lee-
dc.identifier.doi10.1016/j.ejphar.2008.02.072-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00068-
dc.contributor.localIdA01923-
dc.contributor.localIdA02270-
dc.contributor.localIdA03301-
dc.contributor.localIdA03996-
dc.contributor.localIdA04343-
dc.contributor.localIdA04369-
dc.contributor.localIdA04456-
dc.contributor.localIdA00830-
dc.contributor.localIdA00641-
dc.contributor.localIdA03373-1-
dc.relation.journalcodeJ00842-
dc.identifier.eissn1879-0712-
dc.identifier.pmid18387604-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0014299908001714-
dc.subject.keywordMagnesium lithospermate B-
dc.subject.keywordVascular smooth muscle cell-
dc.subject.keywordPlatelet-derived growth factor-
dc.subject.keywordReactive oxygen species-
dc.subject.keywordNeointimal formation-
dc.contributor.alternativeNameKang, Eun Seok-
dc.contributor.alternativeNameKim, Soo Hyun-
dc.contributor.alternativeNameKim, Eun Hee-
dc.contributor.alternativeNameSeo, Hye Sun-
dc.contributor.alternativeNameAhn, Chul Woo-
dc.contributor.alternativeNameLee, Hyun Chul-
dc.contributor.alternativeNameLim, So Yeon-
dc.contributor.alternativeNameCha, Bong Soo-
dc.contributor.alternativeNameHur, Kyu Yeon-
dc.contributor.alternativeNameHeo, Ji Hoe-
dc.contributor.alternativeNameHwang, Ki Chul-
dc.contributor.affiliatedAuthorKang, Eun Seok-
dc.contributor.affiliatedAuthorSeo, Hye Sun-
dc.contributor.affiliatedAuthorAhn, Chul Woo-
dc.contributor.affiliatedAuthorLee, Hyun Chul-
dc.contributor.affiliatedAuthorCha, Bong Soo-
dc.contributor.affiliatedAuthorHur, Kyu Yeon-
dc.contributor.affiliatedAuthorHeo, Ji Hoe-
dc.contributor.affiliatedAuthorHwang, Ki Chul-
dc.contributor.affiliatedAuthorKim, Eun Hee-
dc.contributor.affiliatedAuthorKim, Soo Hyun-
dc.contributor.affiliatedAuthorLim, So Yeon-
dc.rights.accessRightsnot free-
dc.citation.volume586-
dc.citation.number1-3-
dc.citation.startPage226-
dc.citation.endPage233-
dc.identifier.bibliographicCitationEUROPEAN JOURNAL OF PHARMACOLOGY, Vol.586(1-3) : 226-233, 2008-
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurology (신경과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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