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Effect of propofol on calcium homeostasis in hypoxia-reoxygenated neonatal rat cardiomyocytes

 Hyun-Soo Kim  ;  Woo-Chul Chang  ;  Ki-Chul Hwang  ;  In-Geol Choi  ;  Wyun-Kon Park 
 European Journal of Pharmacology, Vol.594(1-3) : 139-145, 2008 
Journal Title
 European Journal of Pharmacology 
Issue Date
Anesthetics, Intravenous/pharmacology* ; Animals ; Animals, Newborn ; Apoptosis/drug effects ; Blotting, Western ; Calcium/metabolism* ; Calcium-Binding Proteins/biosynthesis ; Calcium-Binding Proteins/genetics ; Caspase 3/metabolism ; Cell Hypoxia/drug effects* ; Cell Survival/drug effects ; Gene Expression/drug effects ; Homeostasis/drug effects* ; In Situ Nick-End Labeling ; Male ; Microscopy, Confocal ; Myocytes, Cardiac/drug effects* ; Nerve Tissue Proteins/biosynthesis ; Nerve Tissue Proteins/genetics ; Propofol/pharmacology* ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction/drug effects
Propofol ; Ca2+ homeostasis ; Hypoxia-reoxygenation ; Cardiomyocytes
Intracellular Ca2+ overload induced by hypoxia-reoxygenation alters Ca2+ homeostasis, which plays an important role in myocardial cell injury. Even though propofol is known as a radical scavenger with Ca2+ channel blocking properties, little is known about cardioprotective effect associated with Ca2+ homeostasis in cardiomyocytes. In the present study, we showed that propofol protects cardiomyocytes against hypoxia-reoxygenation injury. In propofol-treated cardiomyocytes, we observed a decrease in the expression of pro-apoptotic protein Bax, cytochrome c, caspase-3 activation and intracellular Ca2+ content. We also found that propofol treatment enhanced expression of anti-apoptotic protein Bcl-2 and activation of ERK concerned with survival. Propofol attenuated alterations of genes involving Ca2+-regulatory mechanism and significantly modulated abnormal changes of SERCA2a genes in hypoxia-reoxygenated neonatal cardiomyocytes. These results suggest that propofol modulates the expression of genes involved in Ca2+ homeostasis, thereby producing cardioprotective effects through a reduction in apoptotic cell death.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Soo(김현수)
Park, Wyun Kon(박윤곤)
Chang, Woo Chul(장우철)
Hwang, Ki Chul(황기철)
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