1 531

Cited 68 times in

Functional recovery after the transplantation of neurally differentiated mesenchymal stem cells derived from bone barrow in a rat model of spinal cord injury

 Sung-Rae Cho  ;  Yong Rae Kim  ;  Hoi-Sung Kang  ;  Sun Hee Yim  ;  Chang-il Park  ;  Yoo Hong Min  ;  Bae Hwan Lee  ;  Ji Cheol Shin  ;  Jong-Baeck Lim 
 CELL TRANSPLANTATION, Vol.18(12) : 1359-1368, 2009 
Journal Title
Issue Date
Animals ; Bone Marrow Cells/cytology* ; Bromodeoxyuridine/metabolism ; Cell Differentiation ; Disease Models, Animal ; Female ; Glial Fibrillary Acidic Protein/metabolism ; Immunohistochemistry ; Mesenchymal Stem Cell Transplantation* ; Mesenchymal Stromal Cells/cytology* ; Motor Activity ; Myelin Basic Protein/metabolism ; Nervous System/cytology ; Rats ; Rats, Sprague-Dawley ; Recovery of Function ; Spinal Cord Injuries/therapy*
Spinal cord injury ; Transplantation ; Mesenchymal stem cells ; Neural differentiation ; Functional recovery
This study was designed to investigate functional recovery after the transplantation of mesenchymal stem cells (MSCs) or neurally differentiated MSCs (NMSCs) derived from bone marrow in a rat model of spinal cord injury (SCI). Sprague-Dawley rats were subjected to incomplete SCI using an NYU impactor to create a free drop contusion at the T9 level. The SCI rats were then classified into three groups; MSCs, NMSCs, and phosphate-buffered saline (PBS)-treated groups. The cells or PBS were administrated 1 week after SCI. Basso-Beattie-Bresnahan (BBB) locomotor rating scores were measured at 1-week intervals for 9 weeks. Somatosensory evoked potentials (SSEPs) and motor evoked potentials (MEPs) were also recorded 8 weeks after transplantation. While transplantation of MSCs led to a clear tendency of motor recovery, NMSC-treated rats had significantly improved BBB scores and showed significantly shortened initial latency, N1 latency, and P1 latency of the SSEPs compared to PBS controls. In addition, 5-bromo-2-deoxyuridine (BrdU)-prelabeled MSCs costained for BrdU and glial fibrillary acidic protein (GFAP) or myelin basic protein (MBP) were found rostrally and caudally 5 mm each from the epicenter of the necrotic cavity 4 weeks after transplantation. These results suggest that neurally differentiated cells might be an effective therapeutic source for functional recovery after SCI.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
Yonsei Authors
Min, Yoo Hong(민유홍) ORCID logo https://orcid.org/0000-0001-8542-9583
Park, Chang Il(박창일)
Shin, Ji Cheol(신지철) ORCID logo https://orcid.org/0000-0002-1133-1361
Lee, Bae Hwan(이배환) ORCID logo https://orcid.org/0000-0003-4719-9021
Lim, Jong Baeck(임종백) ORCID logo https://orcid.org/0000-0003-0419-0422
Cho, Sung-Rae(조성래) ORCID logo https://orcid.org/0000-0003-1429-2684
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.