166 283

Cited 0 times in

압력 저속 냉동 방법의 쥐 치아 치주인대세포 보존 효율 평가

Other Titles
 THE EFFICACY OF PROGRAMMED CRYO-PRESERVATION UNDER PRESSURE IN RAT PERIODONTAL LIGAMENT CELLS 
Authors
 이영은  ;  김의성  ;  김진  ;  한승훈  ;  이승종 
Citation
 Journal of Korean Academy of Operative Dentistry (대한치과보존학회지), Vol.34(4) : 356-363, 2009 
Journal Title
 Journal of Korean Academy of Operative Dentistry (대한치과보존학회지) 
ISSN
 1225-0864 
Issue Date
2009
Keywords
periodontal ligament cell ; MTT ; WST-1 ; viability ; pressure ; cryo-preservation
Abstract
The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at 4℃for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in $37^{\circ}C$water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group. By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth
Files in This Item:
T200902465.pdf Download
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eui Seong(김의성) ORCID logo https://orcid.org/0000-0003-2126-4761
Kim, Jin(김진)
Lee, Seung Jong(이승종)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104215
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links