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압력 저속 냉동 방법의 쥐 치아 치주인대세포 보존 효율 평가
DC Field | Value | Language |
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dc.contributor.author | 김의성 | - |
dc.contributor.author | 김진 | - |
dc.contributor.author | 이승종 | - |
dc.date.accessioned | 2015-04-24T16:48:39Z | - |
dc.date.available | 2015-04-24T16:48:39Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1225-0864 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104215 | - |
dc.description.abstract | The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at 4℃for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in $37^{\circ}C$water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group. By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 356~363 | - |
dc.relation.isPartOf | Journal of Korean Academy of Operative Dentistry (대한치과보존학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 압력 저속 냉동 방법의 쥐 치아 치주인대세포 보존 효율 평가 | - |
dc.title.alternative | THE EFFICACY OF PROGRAMMED CRYO-PRESERVATION UNDER PRESSURE IN RAT PERIODONTAL LIGAMENT CELLS | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Pathology (구강병리학) | - |
dc.contributor.googleauthor | 이영은 | - |
dc.contributor.googleauthor | 김의성 | - |
dc.contributor.googleauthor | 김진 | - |
dc.contributor.googleauthor | 한승훈 | - |
dc.contributor.googleauthor | 이승종 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00833 | - |
dc.contributor.localId | A02925 | - |
dc.contributor.localId | A01009 | - |
dc.relation.journalcode | J01488 | - |
dc.subject.keyword | periodontal ligament cell | - |
dc.subject.keyword | MTT | - |
dc.subject.keyword | WST-1 | - |
dc.subject.keyword | viability | - |
dc.subject.keyword | pressure | - |
dc.subject.keyword | cryo-preservation | - |
dc.contributor.alternativeName | Kim, Eui Seong | - |
dc.contributor.alternativeName | Kim, Jin | - |
dc.contributor.alternativeName | Lee, Seung Jong | - |
dc.contributor.affiliatedAuthor | Kim, Eui Seong | - |
dc.contributor.affiliatedAuthor | Lee, Seung Jong | - |
dc.contributor.affiliatedAuthor | Kim, Jin | - |
dc.citation.volume | 34 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 356 | - |
dc.citation.endPage | 363 | - |
dc.identifier.bibliographicCitation | Journal of Korean Academy of Operative Dentistry (대한치과보존학회지), Vol.34(4) : 356-363, 2009 | - |
dc.identifier.rimsid | 56118 | - |
dc.type.rims | ART | - |
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