국내 B형 간염에 의한 간암 초기 환자에서의 혈장 DNA p53 유전자 Arg72Pro
다형성 및 exon 7 codon 249 돌연변이
Other Titles
Arg72Pro Polymorphism and Exon 7 Codon 249 Mutation of
Plasma DNA p53 Gene in Early Hepatocellular Carcinoma
Patients with Hepatitis B Virus Infection in a Korean population
Authors
Yongjung Park ; Jong-Han Lee ; Eun Young Lee ; Hyon-Suk Kim
Citation
Journal of Laboratory Medicine and Quality Assurance (임상검사와 정도관리), Vol.32(2) : 255-262, 2010
Hepatocellular carcinoma ; p53 ; Hepatitis B virus ; Polymorphism
Abstract
Background: This study was performed to investigate on the genotypic frequencies
of p53 Arg72Pro polymorphism and the prevalence of p53 codon 249 mutation in
hepatocellular carcinoma patients.
Methods: Plasma DNAs were extracted from the samples of 44 early HCC cases,
24 chronic B-viral hepatitis patients and 27 healthy individuals. Serum levels of
AFP, PIVKA-II, and HBV DNA-positive rates among the study groups were also
compared. PCR-based restriction fragment length polymorphism method was used
to determine p53 Arg72Pro genotype and to detect codon 249 mutation.
Results: Serum AFP and PIVKA-II level, Edmondson grade, tumor size and
frequency of HBV DNA-positivity among HCC group according to Arg72Pro
genotypes showed no statistically significant difference. The frequencies of
Arg72Pro genotypes (Arg/Arg, Arg/Pro, Pro/Pro) were respectively as follows:
34.1%, 47.7%, 18.2% in HCC group; 29.2%, 54.2%, 16.7% in hepatitis group;
29.6%, 55.6%, 14.8% in control group. Pro homozygote genotype had a higher
risk for developing HCC by adjusted OR (1.529, 95% CI 0.325-7.193), but not
statistically significant (P=0.591). No codon 249 mutation was found among 44
HCC cases.
Conclusions: Pro homozygote was around 16% in all study groups, and did not
statistically increase risks to developing HCC. We suggest that Arg72Pro
polymorphism of p53 gene is not a significant risk factor in early
hepatocarcinogenesis