0 398

Cited 5 times in

Synthesis of aminated polysorbate 80 for polyplex-mediated gene transfection

Authors
 Eun-Kyung Lim  ;  Jaemoon Yang  ;  Jin-Suck Suh  ;  Yong-Min Huh  ;  Seungjoo Haam 
Citation
 BIOTECHNOLOGY PROGRESS, Vol.26(6) : 1528-1533, 2010 
Journal Title
BIOTECHNOLOGY PROGRESS
ISSN
 8756-7938 
Issue Date
2010
MeSH
Amination ; Antineoplastic Agents/chemical synthesis* ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Cell Survival/drug effects ; DNA/chemistry* ; Drug Carriers/chemical synthesis* ; Drug Carriers/chemistry ; Drug Carriers/pharmacology ; Drug Screening Assays, Antitumor ; Gene Transfer Techniques* ; Green Fluorescent Proteins/chemistry ; HeLa Cells ; Humans ; Materials Testing ; Molecular Structure ; Particle Size ; Plasmids/chemistry ; Polysorbates/chemical synthesis* ; Polysorbates/chemistry ; Polysorbates/pharmacology ; Structure-Activity Relationship ; Surface Properties ; Transfection
Abstract
To develop novel gene delivery carriers, aminated polysorbate 80 (P80-NH(2)) was synthesized with strong positively charged properties through the introduction of three amine groups. The resulting P80-NH(2) and DNA polyplex exhibited superb condensation abilities due to the high densities of positively charged amines groups. Size and surface charge of polyplex were shown to be well suited for cellular internalization. In addition, the P80-NH(2) /DNA polyplex demonstrated acceptable transfection efficiency in HeLa cells and was nontoxic relative to the conventional 25-kDa polyethyleneimine system.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/btpr.475/abstract
DOI
10.1002/btpr.475
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102669
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links