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Overexpression of phosphoinositide-3-kinase class II alpha enhances mesenchymal stem cell survival in infarcted myocardium.

 Lucy Youngmin Eun  ;  Byeong-Wook Song  ;  Min-Ji Cha  ;  Heesang Song  ;  Il-Kwon Kim  ;  Eunmi Choi  ;  Woochul Chang  ;  Soyeon Lim  ;  Eun Ju Choi  ;  Onju Ham  ;  Se-Yeon Lee  ;  Ki Hyun Byun  ;  Yangsoo Jang  ;  Ki-Chul Hwang 
 Biochemical and Biophysical Research Communications, Vol.402(2) : 272-279, 2010 
Journal Title
 Biochemical and Biophysical Research Communications 
Issue Date
The efficacy of mesenchymal stem cell (MSC) therapy for myocardial regeneration is limited by the poor survival of stem cells after transplantation into the infarcted heart. To improve the cell survival of MSCs in the infarcted heart, MSCs were genetically engineered to overexpress phosphoinositide-3-kinase class II alpha (PI3K-C2α). PI3K-C2α overexpression increased PI3K expression and the cell viability of MSCs. Furthermore, levels of survival-related phosphorylation were elevated in PI3K-C2α-MSCs. But, the level of apoptotic proteins downregulated and the number of PI-positive cells decreased in PI3K-C2α-MSCs compared to hypoxic MSCs. Nine rats per group had 1×10(6) cells (20 μl PBS) transplanted after myocardial infarction. One week after transplantation, infarct size and area of fibrosis were reduced in the PI3K-C2α-MSC-transplanted group. The number of TUNEL positive cells declined, while the mean microvessel count per field was higher in the PI3K-C2α-MSC group than the MSC-injected group. Heart function was improved in the PI3K-C2α-MSCs group as assessed using a Millar catheter at 3weeks after transplantation. These findings suggest that overexpression of PI3K-C2α in MSCs can assist cell survival and enhance myocardial regeneration.
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1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터)
Yonsei Authors
김일권(Kim, Il Kwon)
송병욱(Song, Byeong Wook)
이세연(Lee, Se Yeon)
임소연(Lim, So Yeon)
장양수(Jang, Yang Soo) ORCID logo https://orcid.org/0000-0002-2169-3112
장우철(Chang, Woo Chul)
차민지(Cha, Min Ji)
최은미(Choi, Eun Mi)
최은주(Choi, Eun Ju)
함온주(Ham, On Ju)
황기철(Hwang, Ki Chul)
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